Molecular nanotechnology: S-layer technology.
Abstract
Crystalline S-layer represent the outermost cell envelope component of many bacteria and archaea. The goals of the present project are the following: definition of continuous cultivation conditions to obtain biomass with defined cell wall properties, preparation of S-layer ultrafiltration membranes, use of S-layers as matrix for the immobilization of biologically active macromolecules and recrystallization of S-layer proteins on solid supports and interfaces.
Bacillus stearothermophilus functionalized surfaces immobilisation matrix nanoparticles scanning force microscopy S-layers Bacillus sphaericus
Publikationen
Heterogeneous charge transfer at biomolecules immobilized on 2D crystalline bacterial surface proteins (S-layers).
Autoren: Neubauer, A., Pum, D., Dieluweit, S., Sleytr, U. B., Pentzien, S., Kautek, W. Jahr: 1998
Conference & Workshop proceedings, paper, abstract
A new approach to calibration standards in the ten-nanometer range: metal coated S-layers.
Autoren: Neubauer, A., Kautek, W., Dieluweit, S., Pum, D., Sara, M., Traher, C., Sleytr, U. B. Jahr: 1997
Conference & Workshop proceedings, paper, abstract
Investigation of the ultrastructure of S-layers by electron and scanning force microscopy.
Autoren: Pum, D., Neubauer, A., Sara, M., Sleytr, U. B. Jahr: 1997
Conference & Workshop proceedings, paper, abstract
Nanostrukturierte amperometrische Biosensoren auf der Basis von Puls-Laser-kontaktierten kristallinen Enzym-Protein-Kompositschichten.
Autoren: Neubauer, A., Pentzien, S., Reetz, S., Kautek, W., Pum, D., Sleytr, U. B. Jahr: 1997
Conference & Workshop proceedings, paper, abstract
A new approach to calibration standards in the ten-nanometer range: metal coated S-layers.
Autoren: Neubauer, A; Kautek, W; Dieluweit, S; Pum, D; Sahre, M; Traher, C; Sleytr, U B Jahr: 1997
Journal articles
Investigation of the ultrastructure of S-layers by electron and scanning force microscopy.
Autoren: Pum, D; Neubauer, A; Sara, M; Sleytr, U B Jahr: 1997
Journal articles
Creation of (bio)interfaces using self assembled 2-D protein crystals (S-layers).
Autoren: Neubauer, A., Pum, D., Sleytr, U. B. Jahr: 1998
Conference & Workshop proceedings, paper, abstract
10-nm range calibration standards by laser-metallization of S-layer templates.
Autoren: Neubauer, A., Kautek, W., Pentzien, S., Reetz, S., Sahre, M., Solomun, T., Korntner, R., Pum, D., Sleytr, U. B. Jahr: 1998
Conference & Workshop proceedings, paper, abstract
Unconventional nanostructuring approaches.
Autoren: Neubauer, A., Hudek, P., Stangl, G., Riedling, K., Pum, D., Fallmann, W., Sleytr, U. B., Kostic, I., Rüdenauer, F. Jahr: 1997
Conference & Workshop proceedings, paper, abstract
Electrochemical deposition through and electron beam deposition on S-layer templates: a step towards calibration standards in the 10-nm range.
Autoren: Neubauer, A; Kautek, W; Pentzien, S; Reetz, S; Sahre, M; Solomun, T; Korntner, R; Pum, D; Sleytr, U B Jahr: 1998
Journal articles
S-layers: fundamentals and application in molecular nanotechnology.
Autoren: Sleytr, U. B., Pum, D. Jahr: 1999
Conference & Workshop proceedings, paper, abstract
Molekulare Nanotechnologie: S-Schicht Technologie.
Autoren: Sleytr, U. B., Sara, M., Pum, D., Weigert, S., Neubauer, A. Jahr: 1998
Forschungsbericht (extern. Auftraggeber)
Molekulare Nanotechnologie: S-Schicht Technologie.
Autoren: Sleytr, U. B., Sara, M., Pum, D., Weigert, S., Neubauer, A. Jahr: 1998
Forschungsbericht (extern. Auftraggeber)
Molekulare Nanotechnologie: S-Schicht Technologie.
Autoren: Sleytr, U. B., Sara, M., Pum, D., Weigert, S., Neubauer, A. Jahr: 1999
Forschungsbericht (extern. Auftraggeber)
Molekulare Nanotechnologie: S-Schicht Technologie.
Autoren: Sleytr, U. B., Sara, M., Pum, D., Weigert, S., Neubauer, A. Jahr: 1999
Forschungsbericht (extern. Auftraggeber)
Optimierung von Biosensoroberflächen durch DUV-strukturierte S-Schichten.
Autoren: Neubauer, A., Küpcü, S., Pum, D., Sara, M., Sleytr, U. B. Jahr: 1999
Conference & Workshop proceedings, paper, abstract
Molecular nanotechnology with 2D-protein crystals.
Autoren: Sleytr, U. B., Pum, D. Jahr: 2000
Conference & Workshop proceedings, paper, abstract
Characterization and application potential of two-dimensional protein crystals (S-layers).
Autoren: Sleytr, U.B. Jahr: 1999
Conference & Workshop proceedings, paper, abstract
Crystalline bacterial cell surface layers (S-layers).
Autoren: Sleytr, U.B., Messner, P. Jahr: 2000
Chapter in collected volumes
Pulsed-laser metal contacting of biosensors on the basis of crystalline enzyme-protein layer composites
Autoren: Neubauer, A; Pentzien, S; Reetz, S; Kautek, W; Pum, D; Sleytr, UB Jahr: 1997
Journal articles
Project staff
Uwe B. Sleytr
Em.O.Univ.Prof. Dipl.-Ing. Dr. Uwe B. Sleytr
uwe.sleytr@boku.ac.at
Project Leader
01.07.1996 - 31.12.1999
Dietmar Pum
Ao.Univ.Prof.i.R. Dipl.-Ing. Dr.techn. Dietmar Pum
dietmar.pum@boku.ac.at
Sub Projectleader
01.07.1996 - 31.12.1999
BOKU partners
External partners
Federal Institute for Materials Research and Testing
Dr. Wolfgang Kautek
partner
Ion Microfabrication Systems Wien
Dr. Hans Löschner
partner
Ludwig Boltzmann Institut für experimentelle und klinische Traumatologie, Lorenz Böhler Hospital
Prof. Dr. H. Redl
partner
University of Bath
Prof. Steven Mann
partner
Danube University of Krems, Center of Biomedical Technology
Prof. Dr. Dieter Falkenhagen
partner
Technical University Vienna, Institut für Allgemeine Elektrotechnik und Elektronik
Prof. Dr. Wolfgang Fallmann
partner