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Characterization of growth and viability of mesenchymal stem cells in a platelet lysate hydrogel

Project Leader
Kasper Cornelia, Project Leader
Duration:
01.10.2019-30.09.2020
Type of Research
Basic Research
Staff
BOKU Research Units
Institute of Cell and Tissue Culture Technologies
Funded by
PL BioScience GmbH, Dennewartstra├če 25-27, 52068 Aachen, Germany
Abstract
3 test series are to be carried out as follows:
- Long-term 3D cell culture with mesenchymal stem cells
The cells are embedded in the hydrogels and cultivated for 3-5 days. Then they are separated and transferred into a new hydrogel. After passing through, cell count and cell viability are determined. The cells are passed at least three times. From this the cumulative doubling is determined as a function of time and passengers. A statistical group comparison is made.

- Isolation of mesenchymal stem cells from umbilical cord and fat tissue in PL matrix
The aim is to test the effect of the strength of the carrier on the migration of mesenchymal stem cells from tissue to the carrier (matrix). The migration is checked by high-frequency microscopy. When migration is observed, the cells are harvested and counted. Migration rate and cell counts are compared.

- Encapsulation of mesenchymal stem cells for bioreactor cultivation
This experiment will test whether a high-strength PL matrix is stable enough to be used in a stirred tank bioreactor. If this is the case, mesenchymal stem cells are encapsulated in beads and cultured for five days in the stirred tank bioreactor at 200 rpm. If the hydrogels are not strong enough, the strength can be increased or the stirring speed reduced. It is checked whether the cells retain their metabolic activity.
Keywords
Stem cell research; Medical biotechnology; Tissue engineering;
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