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Water’s gateway to heaven: 3D imaging and modeling of transient stomatal responses in plant leaves under dynamic environments

Project Leader
Theroux Rancourt Guillaume, Project Leader
Duration:
01.02.2020-31.01.2024
Programme:
Life Sciences
Type of Research
Basic Research
Project partners
Technische Universität Wien, Austria.
Contact person: Walter Kropatsch;
Function of the Project Partner: Partner
Universität Wien, Austria.
Contact person: Ingeborg Lang;
Function of the Project Partner: Partner
Staff
Tholen Daniel, Project Staff
BOKU Research Units
Institute of Botany
Funded by
Vienna Science and Technology Fund, Schlickgasse 3/12, 1090 Wien, Austria
Abstract
Stomata are tiny pores on the surface of plant leaves and play a central role in global water and carbon cycles. The pores cover less than 5% of the leaf area but facilitate the majority of the exchange of gases between the atmosphere and terrestrial vegetation. The opening of stomata is adjusted to provide CO2 for photosynthesis and to limit water loss. This process exhibits transient responses under fluctuating environmental conditions. The speed at which stomata respond influences productivity and water use efficiency of both crops and natural ecosystems. Although stomatal responses are a target for crop improvement, we lack a clear description of the process, impeding its complete mechanistic understanding. Novel temporal 3D imaging can address the need for a better description of stomatal movements. By complementing fast high-resolution X-ray microcomputed tomography (µCT) with fluorescence microscopy, we will provide in vivo 3D imaging of variations in epidermal cell size and shape and its effects on stomatal movements, scaling from subcellular to whole leaf traits. To fully harness the high volume of data generated by µCT, we will develop novel computational methods to automatically segment images and track single 3D cells over time. This project will answer long-standing questions about stomatal movements and will generate basic knowledge on how to improve stomatal responses under dynamic environments in order to increase net productivity and water-use efficiency.
Keywords
Image processing; Plant anatomy; Plant physiology;
Digital image representation; Fluorescence microscopy; Microcomputed tomography; Stomata; Turgor;
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