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FFoQSI: ToxTesting

Subproject of: FFoQSI Blue Area: Smart Technologies - Scientific innovations for sustainable food chains (FFoQSI Blue Area)

Project Leader
Strauss Joseph, Project Leader
Duration:
01.01.2021-31.12.2024
Programme:
COMET - K1-Zentren
Type of Research
Applied Research
Project partners
AIT Austrian Institute of Technology GmbH, Austria.
Function of the Project Partner: Partner
University of Applied Sciences Upper Austria, 4600 Wels, Austria.
Function of the Project Partner: Partner
Veterinärmedizinische Universität Wien, Austria.
Function of the Project Partner: Partner
Staff
Beyer Reinhard, Project Staff
Zehetbauer Franz, Project Staff
BOKU Research Units
Institute of Microbial Genetics
Funded by
Abstract
Most bioactive metabolites, toxins and contaminants in food and feed such as pesticides, mycotoxins, endocrine disruptors, heavy metals, or polycyclic aromatic hydrocarbons (PAHs) are tested individually in different cellular reporter systems to assess their toxicity and from there deduce the acceptable daily intake (ADI) and maximum residue limits (MRL). In reality, all of these compounds do not occur isolated but in different combinations. To assess their combinatorial action and toxicity levels, we are devloping methods in which differet combinations and concentrations of commonly occuring toxins are tested using laboratory automation for high-content screenings. We construct and apply different cellular (fungal) reporter systems that allow monitoring of substance effects on growth, metabolism and epigentic regulation. The results will help to identify crictical combinations that can then be further tested in mammalian cell culture and in vivo toxicity tests.
Keywords
Microbiology; Molecular biology;
bioactive microbial metabolites; epigenetics; high-content screening; combined toxicity; cellular reporter assays;
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