Functionalization of surfaces using S-layer proteins
Abstract
The project aims at the technical exploitation of recrystallized S-layers. Surfaces functionalized with monolayers of crystalline S-proteins are expected to have a high potential in diagnostic applications (immuno assays, Lab-on-chip), development of quantum structures, or calibration standards for scanning probe microscopy (SPM). By coating materials such as silicon, glass, or synthetic polymers with S-layer proteins, a highly ordered, nanostructured interface is intruduced at the material surface. This allows a reproducible binding of molecules to the surface in defined orientation and dense packing order, which is a prerequisit for the controlled assembly of supramolecular structures (e.g. biochemical layers in microbiosensors, or arrays of metal dots). It is the main objective of this project, to identify potential products in the above mentioned technological fields and develope first prototypes.
Bacillus sphaericus Bacillus stearothermophilus functionalized surfaces immobilisation matrix nanoparticles scanning force microscopy S-layers
Publikationen
S-layer proteins as basic building blocks in a biomolecular construction kit.
Autoren: Sleytr, U.B., Pum, D., Sara, M. Jahr: 2000
Conference & Workshop proceedings, paper, abstract
2D protein crystals (S-layers) as structural basis for a supramolecular construction kit.
Autoren: Sleytr, U.B., Pum, D., Sara, M. Jahr: 2000
Conference & Workshop proceedings, paper, abstract
Funktionalisieren von Oberflächen mit S-Schichten.
Autoren: Neubauer, A., Pum, D., Sleytr, U.B. Jahr: 1999
Forschungsbericht (extern. Auftraggeber)
Funktionalisieren von Oberflächen mittels S-Schichten.
Autoren: Neubauer, A., Pum, D., Sleytr, U.B. Jahr: 2000
Forschungsbericht (extern. Auftraggeber)
Project staff
Dietmar Pum
Ao.Univ.Prof.i.R. Dipl.-Ing. Dr.techn. Dietmar Pum
dietmar.pum@boku.ac.at
Project Leader
01.01.1999 - 30.06.2000
Uwe B. Sleytr
Em.O.Univ.Prof. Dipl.-Ing. Dr. Uwe B. Sleytr
uwe.sleytr@boku.ac.at
Project Staff
01.01.1999 - 30.06.2000
BOKU partners
External partners
Federal Institute for Materials Research and Testing
none
partner
Ion Microfabrication Systems Wien
none
partner
University of Bath
none
partner
Danube University of Krems, Center of Biomedical Technology
none
partner
Technical University Vienna, Institut für Allgemeine Elektrotechnik und Elektronik
none
partner