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Functionalization of surfaces using S-layer proteins

Project Leader

Pum Dietmar, Project Leader

Duration:

01.01.1999-30.06.2000

Type of Research

Technological Development

Project partners

Danube University of Krems, Center of Biomedical Technology, Dr.Karl Dorrek Straße 30, A-3500 Krems, Austria.
Function of the Project Partner: Partner

Federal Institute for Materials Research and Testing, Berlin, Germany.
Function of the Project Partner: Partner

Ion Microfabrication Systems Wien, Wien, Austria.
Function of the Project Partner: Partner

Technical University Vienna, Institut für Allgemeine Elektrotechnik und Elektronik, A-1040 Wien, Austria.
Function of the Project Partner: Partner

University of Bath, BA2 7AY Bath, United Kingdom.
Function of the Project Partner: Partner

Staff

Sleytr Uwe B., Project Staff

Neubauer Angela, Project Staff

BOKU Research Units

Institute of Biophysik

Funded by

Bundesministerium für Wissenschaft und Verkehr, Austria

Abstract

The project aims at the technical exploitation of recrystallized S-layers. Surfaces functionalized with monolayers of crystalline S-proteins are expected to have a high potential in diagnostic applications (immuno assays, Lab-on-chip), development of quantum structures, or calibration standards for scanning probe microscopy (SPM). By coating materials such as silicon, glass, or synthetic polymers with S-layer proteins, a highly ordered, nanostructured interface is intruduced at the material surface. This allows a reproducible binding of molecules to the surface in defined orientation and dense packing order, which is a prerequisit for the controlled assembly of supramolecular structures (e.g. biochemical layers in microbiosensors, or arrays of metal dots). It is the main objective of this project, to identify potential products in the above mentioned technological fields and develope first prototypes.

Keywords

solid state physics; biochemistry; microbiology; biological engineering; ultrastructure research; nanotechnology (nanoengineering); micro-electronics; sensorics;

Bacillus sphaericus; Bacillus stearothermophilus; functionalized surfaces; immobilisation matrix; nanoparticles; scanning force microscopy; S-layers;

Publications

Neubauer, A., Pum, D., Sleytr, U.B. (2000): Funktionalisieren von Oberflächen mittels S-Schichten..

Endbericht im Auftrag des BM für Verkehr, Innovation und Technologie

Sleytr, U.B., Pum, D., Sara, M. (2000): S-layer proteins as basic building blocks in a biomolecular construction kit..

In: Hampp, N., Noll, F. (Eds.): NanoBionics - from Molecules to Applications. 238.WE-Heraeus-Seminar, 10-14 July 2000, Marburg, Germany FullText

Sleytr, U.B., Pum, D., Sara, M. (2000): 2D protein crystals (S-layers) as structural basis for a supramolecular construction kit..

INFMeeting, National Conference on Physics of Matter, Book of Abstracts p.15, Genua, Italy FullText

Neubauer, A., Pum, D., Sleytr, U.B. (1999): Funktionalisieren von Oberflächen mit S-Schichten..

Zwischenbericht im Auftrag des BM für Verkehr, Innovation und Technologie

Vorträge