Molecular analysis of cell expansion in Arabidopsis thaliana
Abstract
During the past years Arabidopsis thaliana has been widely used as model organism in advancing our understanding of the biology of plants. The most exciting progress in the regulation of plant development has been made by the identification of informative mutations in order to assign functions to genes that play a specific role in plant growth and development. A major challenge in the study of plant development is to unravel the molecular basis of morphogenesis. Since cell division and expansion are the major parameters of morphogenesis, their temporal and spatial regulation leads to the characteristic form of plant organs. Although cell expansion is fundamental to establish cell size, shape and polarity, little is known about the molecular mechanisms which control the orientation and the extent of this morphogenic parameter. Using Arabidopsis thaliana, mutants with a root and hypocotyl defect in cell expansion were identified by the applicant. 22 of them are alleles of pom1 and all fail to control the extent and orientation of cell expansion of the two root tissues, epidermis and cortex. Moreover, in pom1 mutants the cell volume of the affected root tissues is significantly greater than that of wild type cells which demonstrates its role in regulating the extent of cell expansion. The defect in root and hypocotyl cell expansion can be modulated by different growth conditions. Hence, pom1 mutants belong to the group of conditional expansion mutants. This project aims to characterise the POM1 gene expression on a transcriptional and translational level. Furthermore, the subcellular localisation of the POM1 gene product will be studied in detail. It is expected that different growth conditions may alter the POM1 activity. The proposed experiments aim to differentiate between a transcriptional, translational or post-translational regulation. To isolate further components of the regulatory pathway of cell expansion we will clone an enhancer of pom1 - the EPO gene - which we isolated in a previous project. Genetic and phenotypic analyses indicate that EPO substitutes for a part of the POM1 function and that the POM1 gene counterbalances the function of the mutated EPO gene. Characterisation of the two cell expansion regulators will be a major contribution toward the understanding of the organ and cell type specific cell volume and polarity control.
Arabidopsis thaliana root morphogenesis
Publikationen
The SHORT-ROOT gene controls radial patterning of the Arabidopsis root through radial signaling.
Autoren: Helariutta, Y; Fukaki, H; Wysocka-Diller, J; Nakajima, K; Jung, J; Sena, G; Hauser, MT; Benfey, PN Jahr: 2000
Journal articles
Histochemical analysis of root meristem activity in Arabidopsis thaliana using a cyclin:GUS (ß-glucuronidase) marker line.
Autoren: Hauser, M.-T., Bauer, E. Jahr: 2000
Journal articles
Identification and Characterization of the ARIADNE Gene - Family in Arabidopsis. A Group of Putative E3 Ligases
Autoren: Mladek, C., Guger, K., Hauser, M.-T. Jahr: 2003
Journal articles
Two new loci, PLEIADE and HYADE, implicate organ-specific regulation of cytokinesis in Arabidopsis.
Autoren: Müller, S; Fuchs, E; Ovecka, M; Wysocka-Diller, J; Benfey, PN; Hauser, MT Jahr: 2002
Journal articles
APL regulates vascular tissue identity in Arabidopsis.
Autoren: Bonke, M; Thitamadee, S; Mähönen, AP; Hauser, MT; Helariutta, Y Jahr: 2003
Journal articles
EPO and the regulation of cell expansion and cellulose synthesis in Arabidopsis.
Autoren: Mansfeld, A, Sigmund, H, Hauser, M-T Jahr: 2003
Conference & Workshop proceedings, paper, abstract
Towards the functional characterization of the ARIADNE-RING finger gene family in Arabidopsis thaliana.
Autoren: Mladek, C and Hauser, M-T Jahr: 2002
Conference & Workshop proceedings, paper, abstract
Characterization of POM2, a gene involved in regulating cell expansion in Arabidopsis thaliana.
Autoren: Li, E and Hauser, M-T Jahr: 2002
Conference & Workshop proceedings, paper, abstract
Structure and differential expression of the ARIADNE-RING finger gene family in Arabidopsis thaliana.
Autoren: Mladek, C and Hauser, M-T () Jahr: 2002
Conference & Workshop proceedings, paper, abstract
Regulation der Zellgröße und des DNA-Gehaltes in Wurzelgeweben von Arabidopsis thaliana
Autoren: Guger, K. Jahr: 2002
Master / Diploma Thesis
Characterization and Isolation of POM2 - a gene involved in the regulation of cell expansion in Arabidopsis thaliana
Autoren: Eryang, L Jahr: 2003
Master / Diploma Thesis
POM2 impairs arrangement of the cytoskeleton and cell all deposition.
Autoren: Bringmann M, Persson S, Li E, Hauser M-T Jahr: 2009
Conference & Workshop proceedings, paper, abstract
The Plant Glycosyl-transferase 64 (GT64) family: in search of a function. Plant Polysaccharides
Autoren: Edvardsson E, Singh SK, Yun M-S, Mansfeld A, M-T Hauser, Marchant A Jahr: 2010
Chapter in collected volumes
Subcellular localization of the HYADE gene product in root tips of Arabidopsis thaliana
Autoren: Pritz C, Winter V, Foissner I, Hauser M-T Jahr: 2010
Conference & Workshop proceedings, paper, abstract
r1r2r3-myb proteins acting as transcriptional repressors for G2/M phase-specific genes
Autoren: Kosuke K, Doonan J, Demura T, Hauser M-T, Pignocchi C, Doonan JH, Ito M Jahr: 2010
Conference & Workshop proceedings, paper, abstract
Amsh3 is a deubiuquitinating enzyme required for vacuole biogenesis
Autoren: Isono E, Katsiarimpa A, Mueller I, Stierhof JD, Schlager N, Hauser M-T, Schwechheimer C Jahr: 2010
Conference & Workshop proceedings, paper, abstract
Mutations in CTL1 (Chitinase-Like 1)/POM1 reduce CESA activity and affects cell wall architecture.
Autoren: Sánchez-Rodríguez C, Bauer S, Hematy K, Ibáñez AB, Aichinger E, Vodermaier V, Hauser M-T, Perssonn S Jahr: 2010
Conference & Workshop proceedings, paper, abstract
Project staff
Marie-Theres Hauser
Ao.Univ.Prof. Dipl.-Ing. Dr.nat.techn. Marie-Theres Hauser
marie-theres.hauser@boku.ac.at
Tel: +43 1 47654-94240
Project Leader
01.07.2000 - 30.06.2004