CE and CE-ICP-SFMS in Rhizosphere Research
Abstract
The understanding of fundamental mechanisms of metal uptake in plants is crucial to the progress in phytoremediation research. The proposed project focuses on the specific problem of metal complexation in the plant root environment (rhizosphere). Root exudates, i. e. substances leaking (passively) from intact cells along a concentration gradient have been proposed to be involved in many processes involved in the rhizosphere, including nutrient acquisition metal mobilization and detoxification because of their capacity to complex metals. A full assessment of their role in these processes, also with respect to hyperaccumulation, however can not be determined unless the exact mechanisms of plant release and the fate of these compounds in the soil are more fully understood. In the submitted project the problem will be addressed by a interdisciplinary approach: First, adequate analytical techniques for studying of metal species will be developed. Analytical methodological work will be carried out in order to determine potential ligands in real samples i. e. soil solutions by CE. CE is an attractive technique for analysis of soil solutions because of its very low volume requirements, favorable detection limits and limited problems with complex matrices, such as high DOC contents. CE-ICP-MS, a novel analytical tool, will be employed for the first time for speciation analysis in exudates released by plant roots. Due to both the advantageous sensitivity of ICP-MS and the minor effect of a CE system on the distribution of chemical species, this combination is ideal for element speciation studies. Goal of the research is to perform metal speciation strictly avoiding potential changes of the species during the separation step. CE-ICP-SFMS methods hold great promise for separating potential ligands, free metals in different oxidation states and the metal complexes utilizing both on-line UV detection and ICP-SMS detection. On the other side the research will focus on development of rhizobox model systems, which allow to simulate the soil situation and sampling of root exudates. Moreover the novel rhizobox design will provide experimental data on the spatial distribution of exudation pattern in the root compartment. For the first time information of whether metals are present in different chemical forms in the rhizosphere will be accessible on a experimental scale. By studying three different plants, namely the Ni hyperaccumulating Thlaspi goesingense, the Zn hyperaccumulating Thlaspi caerulescens and the non¿accumulating Thlaspi arvense the project aims at a better understanding of hyperaccumulation processes in the rhizosphere.
rhizosphere ICP-MS CE
Publikationen
CE-ICP-SFMS and HPIC-ICP-SMFS for arsenic speciation in soil solution and water extracts.
Autoren: Koellensperger, G., Nurmi, K., Hann, S., Stingeder, G., Fitz, W.J., Wenzel, W.W. Jahr: 2002
Journal articles
Novel micro suction cup design for sampling soil solution at defined distances from roots
Autoren: Puschenreiter, M., Wenzel, W.W., Wieshammer, G., Fitz, W.J., Wieczorek, S., Kanltsar, K., Köllensperger, G. Jahr: 2005
Journal articles
Mitarbeiter*Innen
Gunda Köllensperger
Ao.Univ.Prof. Dipl.-Ing. Dr.nat.techn. Gunda Köllensperger
gunda.koellensperger@boku.ac.at
Project Leader
01.08.2001 - 31.07.2005
Walter Wenzel
Univ.Prof. Dipl.-Ing. Dr.nat.techn. Walter Wenzel
walter.wenzel@boku.ac.at
Tel: +43 1 47654-91143, 91161
Project Staff
01.08.2001 - 31.07.2005
BOKU Partner
Externe Partner
Austrian systems research GmbH, Environmental Planning Department
keiner
partner