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Molecular regulation of cytokinesis during plant development

Project Leader
Hauser Marie-Theres, Project Leader
Duration:
01.09.2003-31.08.2007
Type of Research
Basic Research
Staff
Wagner Vera, Project Staff
Werner Ulrike, Project Staff (bis 31.03.2006)
Torres Acosta Juan Antonio, Project Staff (bis 30.09.2014)
Schlager Nicole, Project Staff
BOKU Research Units
Institute of Molecular plant biology
Funded by
Fonds zur Förderung der wissenschaftlichen Forschung (FWF) , Sensengasse 1, 1090 Wien, Austria
Abstract
Cell division is one key parameter of plant growth and development. In general, it consists of nuclear division (karyokinesis) followed by the division of the cytoplasm (cytokinesis). In proliferating cells, cells cycle between cell division and a phase where doubling of the genome occurs (interphase). This cell cycle is regulated by the orchestrated action of rather conserved genes and their activities are indispensible in making the next phase possible. Deregulation of the cell cycle may lead to hyperproliferation, tumour formation or cell death.
The cytoskeleton and in particular the dynamic microtubules play an essential role during cell cycle. In plants, they form four distinct arrays. In interphase, the cortical microtubules array is involved in the alignment of cellulose microfibrills and cell expansion. At the onset of mitosis, the preprophase band defines the plane of cell division. In mitosis, the spindle separates chromosomes and during cytokinesis the phargmoplast directs vesicles with cell wall material to the equatorial plane to form the new cell wall. Microtubule-associated proteins (MAPs) are important regulators of the structure and dynamic of these microtubule assemblies.
The objective of the project is to determine the molecular mechanisms regulating cytokinesis. In preparatory investigations we identified cytokinesis mutants (hyade and pleiade; Müller et al., 2002) in the model plant Arabidopsis thaliana, which hold great promises for this goal.
The proposal is divided in three main tasks focusing on the molecular, cell biological and biochemical analyses of the HYADE and PLEIADE genes and gene products. The third part concentrates on the functional characterization of the PLEIADE/MAP65 gene family.
Due to the molecular basis of the mutants and their homology to proteins of other eukaryotes, the results may lead to a fundamental understanding of the last steps during cell division. And although plants are thought to have developed a specific solution in separating their daughter cells, the basic mechanism of controlling this process may be proven to be similar.
Publications

** Herrmann, A; Livanos, P; Lipka, E; Gadeyne, A; Hauser, MT; Van Damme, D; Muller, S Dual localized kinesin-12 POK2 plays multiple roles during cell division and interacts with MAP65-3.

EMBO REP. 2018; 19(9): WoS PubMed FullText FullText_BOKU

Isono E, Katsiarimpa A, Mueller I, Stierhof JD, Schlager N, Hauser M-T, Schwechheimer C (2010): Amsh3 is a deubiuquitinating enzyme required for vacuole biogenesis. [Poster]
[21th International Conference on Arabidopsis Research, Yokohama, Japan, June 6-10, 2010]

In: ICAR 2010 organizing committee, 21th International Conference on Arabidopsis Research - 2010 and beyond, page 208, abstract 11028

Kosuke K, Doonan J, Demura T, Hauser M-T, Pignocchi C, Doonan JH, Ito M (2010): r1r2r3-myb proteins acting as transcriptional repressors for G2/M phase-specific genes. [Poster]
[21th International Conference on Arabidopsis Research, Yokohama, Japan, June 6-10, 2010]

In: ICAR organizing committee, 21st International Conference on Arabidopsis Research - 2010 and beyond, page 206, poster 11019

Hauser, MT (2008): ESCRT-III dependent protein sorting establishes preprophase band and cell plate positioning.
[5th Tri-National Arabidopsis Meeting, Zürich, SWITZERLAND, SEP 10 – 13, 2008]

In: Sylvain Bischof, Gaelle Messerli, 5th Tri-National Arabidopsis Meeting, Invited Speaker 2, pp 23

Hauser M-T, Schlager N, Winter V, Torres-Acosta JA, Müller S (2006): Towards the Functional Characterization of the Endosomal Sorting Complex Required for Transport (ESCRT) Machinery. .
[15th Congress of the Federation of European Societies of Plant Biology FEPBS, Lyon, 17. – 21.7. 2006]

In: FESPB2006, FESPB2006, http://www.fespb2006.org/, 15th Congress of the Federation of European Societies of Plant Biology FEPBS, 17. – 21.7. 2006, Lyon FullText

Schlager N, Torres-Acosta JA, Hauser M-T (2006): Charakterisierung des ESCRT-III Komplexes in der Modellpflanze Arabidopsis thaliana..
[16. Tagung des Österreichischen Arbeitskreises für Pflanzenphysiologie (ÖAPP) , Mauterndorf, 15. – 18. 6. 2006]

In: ÖAPP, 16. Tagung des Österreichischen Arbeitskreises für Pflanzenphysiologie (ÖAPP) , 15. – 18. 6. 2006, Mauterndorf , http://www.oeapp.org/, 16. Tagung des Österreichischen Arbeitskreises für Pflanzenphysiologie (ÖAPP) , 15. – 18. 6. 2006, Mauterndorf FullText

Schlager N, Winter V, Torres-Acosta JA, Hauser M-T (2006): Characterization of the ESCRT-III complex in Arabidopsis thaliana. .
[3nd Tri-National Arabidopsis Meeting (TNAM), Tübingen, 26. – 29.9. 2006]

In: http://www.eb.tuebingen.mpg.de/tnam2006/tnam2006_ie/index.html, http://www.eb.tuebingen.mpg.de/tnam2006/tnam2006_ie/TNAM06_Program.pdf, 3nd Tri-National Arabidopsis Meeting (TNAM), 26. – 29.9. 2006, Tübingen FullText

Synek L, Schlager N, Elias M, Quentin, M, Hauser M-T, Zarsky V (2006): AtExo70A1, a member of a family of putative Exocyst subunits specifically expanded in land plants, is important for polar growth and plant development..
[3rd EPSO CONFERENCE '06 “Plant Dynamics: from Molecules to Ecosystems” , Visegrád, Hungary, 28.5. – 1. 6. 2006]

In: EPSo, 3rd EPSO Conference '06, http://www.epsoweb.org/catalog/Conf2006.htm http://www.epsoweb.org/catalog/conf2006/Abstract%20Book_Final%20for%20web.pdf, 3rd EPSO CONFERENCE '06 “Plant Dynamics: from Molecules to Ecosystems” , 28.5. – 1. 6. 2006, Visegrád, Hungary FullText

Synek L, Elias M, Drdova E, Quentin M, Hauser M-T, Zarsky V (2005): Analysis of Exo70 exocyst subunits family functions in plants..
[16th International Conference on Arabidopsis Research , Madison, Wisconsin, 15.-19.6, 2005]

In: 16th International Conference on Arabidopsis Research , 16th International Conference on Arabidopsis Research , http://www.arabidopsis.org/news/PosterAbstracts.pdf, 16th International Conference on Arabidopsis Research , 15.-19.6, 2005, Madison, Wisconsin FullText

Vorträge

Marie-Theres Hauser (2011): Does expression correlate with functional importance of gene family members? Examples from the characterization of the MAP65 and ARIADNE families.

, Seminar Series of the ZMBP, OKT 27, 2011, Tübingen

Marie-Theres Hauser (2010): The role of the ESCRTmachinery in plant development .

, GSF- Institut für Biochemische Pflanzenpathologie, Helmholtz Zentrum München, Feb 2,2010, München, Germany

Hauser, M-T (2009): How root development provides insight into the role of mycotoxins and responses to abiotic stressors.

, Max-Planck-Institute for Plant Breeding Research, 28.4.2009, Cologne, Germany

Marie-Theres Hauser (2009): Positioning the plane of cell division in Arabidopsis .

, Universität Münster, Institut für Botanik, July 7 2009, Münster, Germany

Hauser, M-T (2008): ESCRTing the plane of cell division in plants.

, Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, APR 7, 2008, Umeå, SWEDEN

Hauser, M-T (2008): ESCRT-III dependent protein sorting establishes preprophase band and cell plate positioning.

, 5th Tri-National Arabidopsis Meeting, Sept 10– 13, 2008, Zurich, Switzerland

Marie-Theres Hauser (2008): ESCRT-III dependent protein sorting establishes preprophase band and cell plate positioning” .

, 5th Tri-National Arabidopsis Meeting, Sept 10–13, 2008, Zurich, Switzerland

Marie-Theres Hauser (2008): ESCRTing the plane of cell division in plants.

, Umeå Plant Science Centre, Apri 7, 2007, Umeå, Sweden

Hauser, M-T (2007): Zellwandsynthese, Zytoskelettorganisation und Vesikeltransport während der Wurzelmorphogenese.

Eingeladener Vortrag am Institut für Pflanzenwissenschaften, Karl-Franzens-Universität Graz, 19.4. 2007, Graz

Hauser, M-T (2007): MAP65 interaction on plant microtubules and their function.

Plant Systems Biology, VIB2, Meetings & Seminars, 15.2. 2007, Department of Plant Systems Biology, VIB - Ghent University FullText

Hauser, M-T (2007): Functional characterization of the Microtubules Associated Protein 65 Family in Arabidopsis thaliana.

VII International Botanical Microscopy Meeting, 31.3. - 5.4.2007 , Salzburg FullText

Hauser M-T , Baumgartner R, Vordermaier V (2007): Functional characterization of the MAP65 family of microtubules associated proteins..

18th International Conference on Arabidopsis Research, 20.6. - 23. 6. , Beijing FullText

Hauser M-T, Winter V, Nicole Schlager N (2007): Interaction and functional characterization of ESCRT-III complex components of Arabidopsis..

18th International Conference on Arabidopsis Research, 20.6. - 23. 6. , Beijing FullText

Hilscher J, Im UI, Christian Schlötterer C, Hauser M-T (2007): Quantitative genetic analysis of trichome patterning in Arabidopsis thaliana.. [Poster]

4th Tri-National Arabidopsis Meeting (TNAM2007), 12.9. – 15.9. , Vienna FullText

Winter V, Schlager N, Müller S, Torres-Acosta JA, Hauser M-T . (2007): Interaction and functional characterization of ESCRT-III complex components of Arabidopsis.

VII International Botanical Microscopy Meeting, 31.3. – 5.4., Salzburg FullText

WinterV, Schlager N, Gössweiner N, Hauser M-T (2007): Characterization of HYADE, a plant specific ESCRT-III complex component involved in cytokinesis in Arabidopsis.. [Poster]

4th Tri-National Arabidopsis Meeting (TNAM2007), 12.9. – 15.9. , Vienna FullText

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