CHRISTIAN DOPPLER LABORATORY for Mycotoxin Research Mod. ROMER
Abstract
Mycotoxins are toxic secondary metabolites formed by fungi growing on agricultural commodities in the field or during storage. Contamination of cereals and related products with mycotoxins causes food- and feed-borne intoxication in man and farm animals. As a means of mycotoxin prevention, efficient analytical tools for qualitative analysis and quantitation of fungal metabolites as well as appropriate decontamination procedures especially for feed are required. Biological detoxification approaches are increasingly being used to reduce the level of mycotoxins in feeding stuffs. The identification of conjugation or transformation products that arise during microbial degradation processes is of crucial importance during the development of feed additives based on micro organisms, the prime research field of the industrial partners of the proposed study. An important research objective of the Christian Doppler Laboratory is the elucidation of the microbial detoxification metabolisms of the fumonisins FB1, FB2 and zearalenone by means of metabolite identification, characterisation and quantitation using liquid chromatography in combination with triple quadrupole mass spectrometric detection (LC-MS-MS). In addition, conjugation and transformation mechanisms of fumonisins and deoxynivalenol in plant cell cultures will be studied. A major focus of the project is the development of new analytical methods for the determination of trichothecenes, fumonisins and zearalenone. This research work includes novel molecularly imprinted polymers, immunofiltration and new types of sol-gel columns using egg yolk antibodies as well as the development of multimycotoxin methods using LC-MS-MS. The results from the research program of the proposed Christian Doppler Laboratory which will be obtained over a project period of 7 years will provide the basis for the further development of powerful microbiological detoxifying agents in the feed industry. Moreover, tools for rapid and accurate monitoring of mycotoxins and their transformation and conjugation products will become available.
Publikationen
Project staff
Rudolf Krska
Univ.Prof. Dipl.-Ing. Dr.techn. Rudolf Krska
rudolf.krska@boku.ac.at
Tel: +43 1 47654-97301, 97302
Project Leader
01.12.2002 - 30.11.2009