Functional studies of the ARIADNE gene family in Arabidopsis thaliana
Abstract
Recently, we identified and classified the ARIADNE (ARI) protein family in the model plant Arabidopsis thaliana (Mladek et al., 2003; www.arabidopsis.org/info/genefamily/ARIADNE.html). Members of this family share two C3HC4 RING (really interesting genes)-finger motifs, which are separated by a central C6HC domain called the "in between RING-finger" (IBR), B-box or DRIL (double RING finger-linked) domain. Similar proteins are widespread in eukaryotes, including humans. There is increasing evidence that RING-finger proteins mediate ubiquitination of proteins targeted for proteolysis via the 26S proteasome pathway. Furthermore, RING-finger proteins play a central role in determining substrate specificity. Ubiquitin-mediated proteolysis is involved in many aspects of plant development including regulation of hormone signaling, embryogenesis, cell cycle, photomorphogenesis, circadian rhythms, flower development, leaf senescence, disease resistance and wax synthesis. At present, around 30 genes have been assigned to this pathway. Nevertheless, only four are RING-finger proteins and none belongs to the ARI class. Using sequence comparison and phylogenetic analyses we have grouped the 16 AtARI proteins of Arabidopsis into three subclasses. Interestingly, the members of two subclasses are absent in invertebrates and vertebrates, possibly indicating a plant-specific function. The objective of the project is to understand i) the functional role, ii) the biochemical activity and iii) the regulation of some members of the ARI gene family in Arabidopsis. The expression level at diverse developmental stages and different organs has already been characterized in our group (Mladek et al., 2003). Furthermore, promoter:reporter constructs were already generated for half of the members of the ARI family. We also prepared the phenotypic analysis of double and triple mutants by isolating knockouts and generating the required crosses. For three of the AtARI proteins we generated specific peptide antibodies for cell biological and biochemical analyses and produced recombinant proteins in E. coli. With these tools at hands the project will determine the role of ARI proteins in plants.
RING-finger Arabidopsis thaliana ARIADNE proteolysis E3 ligase
Publikationen
The RBR protein family.
Autoren: Eisenhaber B, Chumak N, Eisenhaber F, Hauser M-T Jahr: 2007
Journal articles
AtARI12 is a RING finger E3 ligase involved in resistance to UV-B stress.
Autoren: Mladek C, Hauser M-T Jahr: 2008
Conference & Workshop proceedings, paper, abstract
Interaction partners of ARIADNE E3 Ligases indicates their function in posttranscriptional regulation.
Autoren: Nigam N, MladekC, Torres Acosta J-A, Hauser M-T Jahr: 2008
Conference & Workshop proceedings, paper, abstract
Reactivation of transcriptionally silenced loci by abiotic stress.
Autoren: Popova O, Mladek C, Kiok K, Berlinger M, Luschnig C, Aufsatz W, Hauser M-T, Jonak C Jahr: 2008
Conference & Workshop proceedings, paper, abstract
The Class ARIADNE E3 Ligases and Their Interaction Partners Are Involved in Diverse Stress Responses.
Autoren: Nigam N, MladekC, Torres Acosta J-A, Hauser M-T Jahr: 2009
Conference & Workshop proceedings, paper, abstract
The E3 ligase AtARI12 regulates the adaptation to repetitive UV-B exposures and long-term low temperatures.
Autoren: Lang-Mladek C, Nigam N, Chen H-W, Hauser M-T Jahr: 2009
Conference & Workshop proceedings, paper, abstract
The Arabidopsis E3 ligase ARI12 is involved in post-transcriptional regulation of mRNA
Autoren: Lang-Mladek C, Nigam N, Burey SC, Torres Acosta JA, Hauser M-T Jahr: 2010
Conference & Workshop proceedings, paper, abstract
ARIADNE E3 ligases and their interaction partners mediate abiotic stress responses
Autoren: Nigam N, Lang-Mladek C, Torres Acosta JA, Hauser M-T Jahr: 2010
Conference & Workshop proceedings, paper, abstract
The Arabidopsis E3 ligase AtARI12 is involved in long term adaptation to elevated UV-B radiation
Autoren: Lang-Mladek C, Nigam N, Chen H-W, Hauser M-T Jahr: 2010
Conference & Workshop proceedings, paper, abstract
Transgenerational stability and mechanisms of UV-B induced epigenetic changes in Arabidopsis thaliana.
Autoren: Lang-Mladek C, Popova O, Kiok K, Berlinger M, Rakic B, Jonak C, Luschnig C, Aufsatz W, Hauser M-T Jahr: 2011
Conference & Workshop proceedings, paper, abstract
Natural variation of growth upon exposure to chronic broad range UV-B radiation
Autoren: Hauser, M-T; Xie, L; Neubert, S; Hilscher, J Jahr: 2012
Conference & Workshop proceedings, paper, abstract
Which photoreceptors are involved in the transcriptional regulation of ARIADNE12 upon broad band UV-B radiation?
Autoren: Xie, L; Lang-Mladek, C; Neubert, S; Hauser M-T (2012) Cost Action UV4growth mini-conference. Acclimation to UV-B, Glasgow, Scotland, 1. – 2.5. Jahr: 2012
Conference & Workshop proceedings, paper, abstract
Regulation and function of ARIADNE12 upon UV-B radiation in Arabidopsis.
Autoren: Xie L, Lang-Mladek C, Neubert S, Hauser M-T Jahr: 2012
Conference & Workshop proceedings, paper, abstract
Induction of ARI12 upon broad band UV-B radiation is suppressed by UVR8 and cryptochromes
Autoren: Xie, L., Hauser, M.-T. Jahr: 2012
Journal articles
The role of translation in UV-B induced secondary metabolite biosynthesis.
Autoren: Winkler B, Mewis I, Lang-Mladek C, Baldermann S, Bamberg A, Albert A, Schreiner M, Hauser M-T Jahr: 2014
Conference & Workshop proceedings, paper, abstract
Ecophysiological consequences of UV-B radiation and ozone on Brassicaceae resistance traits.
Autoren: Mewis I, Winkler JB, Albert A, Hauser M-T, Schreiner M, Baldermann S, Zrenner R, Schulz H, Ulrichs C Jahr: 2016
Conference & Workshop proceedings, paper, abstract
Role of the ARIADNE12 ubiquitin E3 ligase and its putative substrate in long term adaptation to UV-B.
Autoren: Hauser M-T, Lisi Xie, Julia Richter, Karolina Słomińska, Lukas Zeh, Serap Afşar, Neha Nigam and Christina Lang-Mladek Jahr: 2018
Conference & Workshop proceedings, paper, abstract
Project staff
Marie-Theres Hauser
Ao.Univ.Prof. Dipl.-Ing. Dr.nat.techn. Marie-Theres Hauser
marie-theres.hauser@boku.ac.at
Tel: +43 1 47654-94240
Project Leader
16.08.2005 - 15.08.2008