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S-layers as versatile building blocks in nanobiotechnology (P18510-B12)

Project Leader
Egelseer Eva Maria, Project Leader
Duration:
01.01.2006-30.06.2009
Type of Research
Basic Research

Further information: http://www.fwf.ac.at/de/abstracts/abstract.asp?L=D&PROJ=P18510

Staff
BOKU Research Units
Institute of Biophysik
Funded by
Fonds zur Förderung der wissenschaftlichen Forschung (FWF) , Sensengasse 1, 1090 Wien, Austria
Abstract
Many bacteria and archaea possess regularly structured protein lattices, so called S-layers as their outermost cell envelope component which completely cover the cell surface during all stages of bacterial growth and division. In gram-positive bacteria, the S-layer subunits recognize distinct types of heteropolysaccharides, termed secondary cell wall polymers (SCWP) as the proper anchoring structure in the rigid cell wall layer. Basically, two main binding mechanisms between S-layer proteins and SCWPs have been described. The first one, which involves so called S-layer-homologous (SLH) domains and pyruvylated SCWPs has been found to be widespread among prokaryotes and is considered as having been conserved in the course of evolution. The second type of binding mechanism which has been described for Geobacillus stearothermophilus wild-type strains, involves SCWPs that contain 2,3-dideoxy-diacetamido mannosamine uronic acid as the negatively charged component and an N-terminal part which does not possess an SLH-domain. The present study will focus on the investigation of the distribution (occurrence) of a probably second type of conserved binding mechanism between S-layer proteins and SCWPs in selected strains of gram-positive bacteria, as observed between the conserved N-terminus of S-layer proteins from G. stearothermophilus wild-type strains and non pyruvylated, mannosamine uronic acid-containing SCWPs. In this context, also the gene cluster responsible for synthesis of the non pyruvylated SCWP in G. stearothermophilus wild-type strains shall be identified. In the G. stearothermophilus wild-type strain PV72/p6, the S-layer protein SbsA was replaced by the S-layer protein SbsB of the variant designated PV72/p2 under non oxygen-limited growth conditions. During the switch from sbsA to sbsB, also the type of SCWP was changed. In the present project, the DNA region surrounding the S-layer gene sbsB shall be sequenced and investigated for the presence of genes responsible for synthesis of the variant specific, pyruvylated SCWP. Furthermore, it will be investigated, if the genes required for synthesis of the variant specific SCWP are also located on the sbsB-carrying megaplasmid before the switch or if they are located elsewhere on the chromosome in the wild-type strain PV72/p6. In the present study, it will also be studied, if G. stearothermophilus wild-type strains for which the development of SLH-carrying S-layer proteins has not yet been observed, have the intrinsic potential for variant formation under non oxygen-limited growth conditions. Since our group has previously demonstrated that S-layers and SCWPs are useful building blocks for nanobiotechnological applications, the present research project should contribute to a better understanding of the molecular mechanism beyond the highly specific interaction between bacterial S-layer proteins and cell wall polysaccharides.
Keywords
biochemistry; microbiology; molecular biology; nanotechnology (nanoengineering);
Bacillaceae; anchoring mechanisms; nanobiotechnology; secondary cell wall plomers; S-layer proteins; S-layer variations;
Publications

Sleytr, U.B., Schuster, B., Egelseer, E.M., Pum, D., Horejs, C.M., Tscheliessnig, R., Ilk, N. (2011): Nanotechnology with S-Layer Proteins as Building Blocks.

In: Horworka, S. (Ed.), Progress in Molecular Biology and Translational Science 103, pp. 277-352; Academic Press, Burlington, US; ISBN 978-0-12-415906-8

Egelseer, E.M., Ilk, N., Pum, D., Messner, P., Schäffer, C., Schuster, B., Sleytr, U.B. (2010): S-Layers, Microbial, Biotechnological Applications.

In: Flickinger, M.C. (Ed.), Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology, pp. 4424-4448; Wiley-VCH Verlag, Weinheim, Germany

Messner, P., Schäffer, C., Egelseer, E.M., Sleytr, U.B. (2010): Occurrence, structure, chemistry, genetics, morphogenesis, and functions of S-layers.

In: H. König, H. Claus, and A. Varma (Eds.), Prokaryotic cell wall compounds - structure and biochemistry, pp. 53-109; Springer-Verlag, Berlin, Germany

Sleytr, U.B., Egelseer, E.M., Ilk, N., Messner, P., Schäffer, C., Pum, D., Schuster, B. (2010): Nanobiotechnological Applications of S-Layers.

In: H. König, H. Claus, A. Varma (Eds.) , Prokaryotic Cell Wall Components – Structure and Biochemistry, pp. 459-481; Springer Verlag, Heidelberg, Germany

** Badelt-Lichtblau, H., Kainz, B., Völlenkle, C., Egelseer, E.M., Sleytr, U.B., Pum, D., Ilk, N. Genetic engineering of thr S-layer protein SbpA of Lysinibacillus sphaericus CCM 2177 for the generation of functionalized nanoarrays.

BIOCONJUGATE CHEM, 20, 895-903; ISSN 1043-1802 WoS PubMed FullText FullText_BOKU

** Ferner-Ortner-Bleckmann, J; Huber-Gries, C; Pavkov, T; Keller, W; Mader, C; Ilk, N; Sleytr, UB; Egelseer, EM The high-molecular-mass amylase (HMMA) of Geobacillus stearothermophilus ATCC 12980 interacts with the cell wall components by virtue of three specific binding regions..

Mol Microbiol. 2009; 72(6):1448-1461 WoS PubMed FullText FullText_BOKU

** Pavkov, T; Egelseer, EM; Tesarz, M; Svergun, DI; Sleytr, UB; Keller, W The structure and binding behavior of the bacterial cell surface layer protein SbsC..

Structure. 2008; 16(8):1226-1237 WoS PubMed FullText FullText_BOKU

** Ilk, N; Egelseer, EM; Ferner-Ortner, J; Kupcu, S; Pum, D; Schuster, B; Sleytr, UB Surfaces functionalized with self-assembling S-layer fusion proteins for nanobiotechnological applications.

COLLOID SURFACE A. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS; 321: 163-167. WoS FullText FullText_BOKU

Egelseer, E.M., Sára, M., Pum, D., Schuster, B., Sleytr, U.B. (2008): Genetically engineered S-layer proteins and S-layer-specific heteropolysaccharides as components of a versatile molecular construction kit for applications in nanobiotechnology.

In: Shoseyov, O., Levy, I. (Eds.) , Nanobiotechnology, pp. 55-86; Humana Press , Totowa, NJ

** Ferner-Ortner, J; Mader, C; Ilk, N; Sleytr, UB; Egelseer, EM High-affinity interaction between the S-layer protein SbsC and the secondary cell wall polymer of Geobacillus stearothermophilus ATCC 12980 determined by surface plasmon resonance technology..

J Bacteriol. 2007; 189(19):7154-7158 WoS PubMed PUBMED Central FullText FullText_BOKU

** Gerstmayr, M; Ilk, N; Schabussova, I; Jahn-Schmid, B; Egelseer, EM; Sleytr, UB; Ebner, C; Bohle, B A novel approach to specific allergy treatment: the recombinant allergen-S-layer fusion protein rSbsC-Bet v 1 matures dendritic cells that prime Th0/Th1 and IL-10-producing regulatory T cells..

J Immunol. 2007; 179(11):7270-7275 WoS PubMed

** Sleytr, UB; Huber, C; Ilk, N; Pum, D; Schuster, B; Egelseer, EM; S-layers as a tool kit for nanobiotechnological applications..

FEMS Microbiol Lett. 2007; 267(2):131-144 WoS PubMed

Pavkov, T., Egelseer, E. M., Hammel, M., Sára, M., Keller, W. (2006): First structure of a bacterial surface layer (S-layer) protein reveals a novel coiled-coil fold.
[Annual Meeting of the ÖGBM, ÖGGGT, ÖGBT & ANGT - Life Science 2006, Salzburg, Austria, 25.09.2006 - 27.09.2006]

In: ÖGBM, ÖGGGT, ÖGBT & ANGT (Eds.), Life Science Meeting 2006, p. 27, Annual Meeting of the ÖGBM, ÖGGGT, ÖGBT & ANGT, September, Salzburg

Vorträge

Kroutil, M., Pavkov, T., Egelseer, E. M., Sára, M., Keller, W. (2006): Biophysical characterization of C-terminal part of S-layer protein SbsC. [Poster]

Joint Annual Meeting of the ÖGBM, ÖGGGT, ÖGBT & ANGT, September, Salzburg

Pavkov, T., Egelseer, E. M., Hammel, M., Sára, M., Keller, W. (2006): First structure of a bacterial surface layer (S-layer) protein reveals a novel coiled-coil fold.

Annual Meeting of the ÖGBM, ÖGGGT, ÖGBT & ANGT, September, Salzburg

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