Determination and Characterisation of Secondary Metabolites of Trichoderma atroviride
Abstract
Determination and Characterisation of Secondary Metabolites of Trichoderma atroviride Trichoderma spp. are filamentous fungi which frequently colonise soil and plant roots. They are potent parasites of phytopathogenic fungi (mycoparasites) and some Trichoderma species are able to induce systemic resistance in plants. Therefore, these fungi are commercially used as biocontrol agents as an alternative to chemical pesticides. The current project is a co-operation with Dr. Susanne Zeilinger of the Institute of Chemical Engineering at the Vienna University of Technology. The analytical part of the project aims at the investigation of the molecular mechanisms of biocontrol and the mycoparasitic properties of T. atroviride. As mycoparasitism involves the production of lytic enzymes and antifungal secondary metabolites by Trichoderma, we will analyse the formation of secondary metabolites of T. atroviride and compare the wild-type strain and different deletion mutants. We want to identify and characterise the molecular structures of these metabolites and investigate the metabolite profiles by GC-MS and LC-MS/MS. We expect that the results of the analytical measurements will help to improve the knowledge of the complex mechanisms involved in mycoparasitism and biocontrol.
Trichoderma mass spectrometry peptaibols secondary metabolites
Publikationen
Project staff
Rainer Schuhmacher
Univ.Prof. Dipl.-Chem. Dr.rer.nat. Rainer Schuhmacher
rainer.schuhmacher@boku.ac.at
Tel: +43 1 47654-97307
Project Leader
01.01.2006 - 31.07.2009
BOKU partners
External partners
Technische Universität Wien
none
coordinator