SFB F37-Fusarium
Abstract
Many filamentous fungi causing plant diseases have the ability to produce toxic secondary metabolites. So far most of the research has focused on compounds accumulating in infected cereals at toxicologically relevant levels for humans and animals consuming contaminated food or feed. The most important mycotoxin producing pathogens in Europe are species of Fusarium which cause head blight of small grain cereals and ear rot of maize. Only polygenically inherited quantitative differences in resistance exist in plant genetic resources, and the molecular basis of quantitative trait loci improving resistance is unknown. The goal of our project is to get an improved understanding of the role of fungal secondary metabolites in disease development, in order to be able to dissect plant resistance components. We will use the tools of modern genomics and metabolomics to change plant breeding from a solely empirical to a knowledge-based science, which should allow breeding of Fusarium resistant cultivars with low mycotoxin content. Our working hypothesis is that necrotrophic fungal pathogens like Fusarium produce a redundant set to metabolites which can suppress defense responses in plants, causing the broad host range. Bioinformatical analysis of the available full genome sequence of Fusarium graminearum revealed that this organism possesses many genes predicted to code for enzymes involved in secondary metabolite biosynthesis (e.g. 15 polyketide synthases, 20 ribosomal peptide synthases and 17 terpenoid synthases). For most of the genes no corresponding metabolites are known. The goal of our project is to use advanced functional genomics and metabolomics tools to identify new Fusarium metabolites suppressing defense responses triggered by the pathogen and purified elicitor proteins of Fusarium. With such compounds at hand we will explore their mode of action in model plants, and investigate the genome-wide transcriptional response in model and crop plants after challenge with toxins or with the pathogen. We will explore if differences exist in the breeding material regarding the ability to metabolize and detoxify (known and new) Fusarium metabolites. Candidate genes involved in detoxification (and supposedly pathogen resistance) will be validated by analysis of loss of function alleles identified by screening a wheat TILLING population, or by overexpression in transgenic wheat. The interdisciplinary collaboration of scientists integrating fungal genomics and bioinformatics, metabolomics, model and crop plant genomics and plant breeding distinguishes this proposal from other ongoing projects.
Publikationen
Towards the establishment of ZON reporters in plants
Autoren: Merz D, Werner U, Berthiller F, Neubert S, Krska R, Adam G, Hauser M-T, Jahr: 2009
Conference & Workshop proceedings, paper, abstract
The Fusarium mycotoxin zearalenone inhibits Hsp90 ATPase activity and is inactivated in planta by glucosylation and sulfatation
Autoren: Torres Acosta, J. A., Berthiller, F., Wiesenberger, G., Mitterbauer, R., Werner, U., Merz, D., Hauser, M.-T. Shams, M., Krska, R., Adam, G. Jahr: 2010
Conference & Workshop proceedings, paper, abstract
Formation of the Biomarker Zearalenone-4-O-Glucuronide by Human UDP-Glucuronosyltransferases and Engineered Yeast
Autoren: W. Schweiger, F. Berthiller, W. Bicker, R. Schuhmacher, R. Krska, H. Mikula, C. Hametner and G. Adam Jahr: 2009
Conference & Workshop proceedings, paper, abstract
The Fusarium toxis β-zearalenol and zearalenone inhibit Hsp90 ATPase activity and are inactivated by glucosylation and sulfatation.
Autoren: J.A. Torres Acosta, F. Berthiller, G. Wiesenberger, R. Mitterbauer, U. Werner, D. Merz, M.-T. Hauser, M. Shams, R. Krska, G.Adam Jahr: 2010
Conference & Workshop proceedings, paper, abstract
Formation of DON-glutathione conjugates: a role for glutathione-S-transferases in DON resistance of barley?
Autoren: G.Adam, F. Berthiller, C. Hametner, S. A. Gardiner, J. Boddu, R. Stupar, G.J. Muehlbauer Jahr: 2010
Conference & Workshop proceedings, paper, abstract
Evidenz für Glutathion-vermittelte Detoxifikation von Deoxynivalenol in Gerste und Bäckerhefe
Autoren: Adam, G; Berthiller, F; Hametner, C; Gardiner, S; Muehlbauer G Jahr: 2010
Journal articles
Ein Wirkungsmechanismus für Zearalenon in Pflanzen: Inhibition der ATPase Aktivität von Hitzeschock-Protein 90
Autoren: Torres Acosta, JA; Wiesenberger, G; Kmiecik, P; Berthiller, F; Shams, M; Krska, R; Adam G Jahr: 2010
Journal articles
Bildung eines nicht-toxischen Metaboliten von Diacetoxyscirpenol durch Kochen von Fusarium kontaminierten Erdäpfeln
Autoren: Shams, M; Mitterbauer, R; Corradini, R; Schuhmacher, R; Krska, R; Adam, G; Berthiller, F Jahr: 2010
Journal articles
Nutzung der Genomsequenz des Modellgrases Brachypodium distachyon zum Studium der UDP-Glucosyltransferase Genfamilie von Getreide
Autoren: Schweiger, W; Kovalsky, P; Boddu, J; Shin, S; Muehlbauer, G; Mayer, K; Berthiller, F; Adam, G Jahr: 2010
Journal articles
Gene expression analysis of related wheat lines with contrasting levels of head blight resistance after Fusarium graminearum inoculation
Autoren: Steiner Barbara, Apinun Limmongkon, Katharina Schiessl, Marc Lemmens, Hayan Jia, Gary Muehlbauer, Alexandra Posekany, David P. Kreil and Hermann Buerstmayr Jahr: 2010
Conference & Workshop proceedings, paper, abstract
Gene expression analysis of related wheat lines with contrasting levels of head blight resistance after Fusarium graminearum inoculation
Autoren: Steiner Barbara, Apinun Limmongkon, Katharina Schiessl, Marc Lemmens, Hayan Jia, Gary Muehlbauer, Alexandra Posekany, David P. Kreil, Hermann Buerstmayr Jahr: 2010
Conference & Workshop proceedings, paper, abstract
Gene expression analysis of related wheat lines with contrasting levels of head blight resistance after Fusarium graminearum inoculation
Autoren: Steiner Barbara, Apinun Limmongkon, Katharina Schiessl, Marc Lemmens, Hayan Jia, Gary Muehlbauer, Alexandra Posekany, David P. Kreil, Hermann Buerstmayr Jahr: 2010
Conference & Workshop proceedings, paper, abstract
Identifying and characterizing barley genes that protect against trichothecenes
Autoren: Shin, S.H., Heinen, S.J., Schweiger, W., Adam, G., Muehlbauer, G.J. Jahr: 2010
Conference & Workshop proceedings, paper, abstract
Isolation and Characterization of a New Less-Toxic Derivative of the Fusarium Mycotoxin Diacetoxyscirpenol after Thermal Treatment
Autoren: Shams, M; Mitterbauer, R; Corradini, R; Wiesenberger, G; Dall'Asta, C; Schuhmacher, R; Krska, R; Adam, G; Berthiller, F Jahr: 2011
Journal articles
Arabidopsis reporter plants for in vivo detection of the mycotoxin zearalenone – preparations for a mutagenesis screen to detect mutants with altered signaling, uptake or metabolization
Autoren: Merz D, Ayad M, Pfaffenbichler N, Adam G, Hauser M-T Jahr: 2010
Conference & Workshop proceedings, paper, abstract
The Fusarium toxins beta-zearalenol and zearalenone inhibit Hsp90 ATPase activity and are inactivated by glucosylation and sulfatation.
Autoren: Torres Acosta JA, Berthiller F, Wiesenberger G, Mitterbauer R, Werner U, Merz D, Hauser M-T, Shams M, Krska R, Adam G Jahr: 2010
Conference & Workshop proceedings, paper, abstract
Production of auxin from tryptamine: a role in virulence of Fusarium graminearum?
Autoren: Bartholomäus, A., Wiesenberger, G., Krska, R., Adam, G., Berthiller, F. Jahr: 2011
Conference & Workshop proceedings, paper, abstract
De-acetylation of 3-ADON and 15-ADON by plant and Fusarium enzymes
Autoren: Schmeitzl, C., Shams, M., Wiesenberger, G., Lemmens, M., Berthiller, F., Adam, G. Jahr: 2011
Conference & Workshop proceedings, paper, abstract
Mechanisms of self resistance to zearalenone in Fusarium graminearum
Autoren: Adam, G., Torres Acosta, J. A.,Berthiller, F., Wiesenberger, G., Mitterbauer, R., Krska, R. Jahr: 2011
Journal articles
Cloning and heterologous expression of candidate DON-inactivating UDP-glucosyltransferases from rice and wheat in yeast.
Autoren: Schweiger, W; Steiner, B; Limmongkon, A; Brunner, K; Lemmens, M; Berthiller, F; Bürstmayr, H; Adam, G Jahr: 2011
Journal articles
Fusarium sulphureum delta-tri101 mutants overproduce the toxic metabolite FS-4
Autoren: Wiesenberger, G., Shams, M., Mitterbauer, R., Hellmer, H., Berthiller, F., Hametner, C., Adam, G. Jahr: 2011
Journal articles
Transcriptomic characterization of Fusarium resistance QTL Fhb1 and Qfhs.ifa-5A in wheat near-isogenic lines
Autoren: Schweiger, W; Steiner, B; Kreil, DP; Berthiller, F; Lemmens, M; Muehlbauer, GJ; Buerstmayr, H Jahr: 2011
Conference & Workshop proceedings, paper, abstract
Reconstruction and cloning of wheat UDP-glucosyltransferases with the putative ability to inactivate the Fusarium toxin deoxynivalenol
Autoren: Teutschl, A; Schweiger, W; Siegwart, G; Sam, E; Steiner, B; Bürstmayr, H; Lemmens, M; Adam G; Jahr: 2011
Conference & Workshop proceedings, paper, abstract
Expression-QTL mapping in wheat to identify genes involved in resistance to Fusarium graminearum
Autoren: Samad-Zamini, M; Schweiger, W; Siegwart, G; Sam, E; Steiner, B; Bürstmayr, H; Lemmens, M; Adam, G; Jahr: 2011
Conference & Workshop proceedings, paper, abstract
Transcriptomic characterization of Fusarium resistnace QTL Fhb1 and Qfhs.ifa-5A in wheat near-isogenic lines
Autoren: Schweiger, W; Steiner, B; Kreil, DP; Berthiller, F; Lemmens, M; Muehlbauer, GJ; Buerstmayr, H; Jahr: 2012
Conference & Workshop proceedings, paper, abstract
Transgenic Arabidopsis thaliana expressing a barley UDP-glucosyltransferase exhibit resistance to the mycotoxin deoxynivalenol
Autoren: Shin, S; Torres-Acosta, JA; Heinen, SJ; McCormick, S; Lemmens, M; Paris, MPK; Berthiller, F; Adam, G; Muehlbauer, GJ Jahr: 2012
Journal articles
Expression-QTL Mapping in Wheat to Identify Regulatory Hotspots Involved in the Resistance To Fusarium graminearum
Autoren: Samad-Zamini, M; Schweiger, W; Sam, E; Siegwart, G; Steiner, B; Lemmens, M; Buerstmayr H Jahr: 2012
Conference & Workshop proceedings, paper, abstract
Transcriptomic Characterization of the Fusarium Resistance QTL Fhb1 and Qfhs-ifa.5A.
Autoren: Schweiger, W; Steiner, B; Ametz, C; Siegwart, G; Wiesenberger, G; Berthiller, F; Lemmens, M; Jia, H; Adam, G; Muehlbauer, GJ; Kreil, DP; Buerstmayr, H Jahr: 2012
Conference & Workshop proceedings, paper, abstract
Transcriptome Sequencing of Fusarium Challenged Wheat Near Isogenic Lines: A Comparison of Methods
Autoren: Siegwart, G; Ametz, C; Zamini, M; Steiner, B; Schweiger, W; Buerstmayr, H Jahr: 2012
Conference & Workshop proceedings, paper, abstract
Functional Genomics of UDP-Glucosyltransferases: Heterologous Expression in Yeast to Test for Deoxynivalenol Detoxification Capability of Candidate Genes
Autoren: Schweiger, W; Kovalsky Paris MP; Wiesenberger, G; Berthiller, F; Lemmens, M; Shin, S; Torres Acosta, JA; Muehlbauer, GJ; Adam, G Jahr: 2012
Conference & Workshop proceedings, paper, abstract
Expression-QTL Mapping in Wheat to Identify Regulatory Hotspots Involved in the Resistance To Fusarium graminearum
Autoren: Samad-Zamini, M; Schweiger, W; Sam, E; Siegwart, G; Steiner, B; Lemmens, M; Buerstmayr H Jahr: 2012
Conference & Workshop proceedings, paper, abstract
Transcriptomic Characterization of the Fusarium Resistance QTL Fhb1 and Qfhs-ifa.5A.
Autoren: Schweiger, W; Steiner, B; Ametz, C; Siegwart, G; Wiesenberger, G; Berthiller, F; Lemmens, M; Jia, H; Adam, G; Muehlbauer, GJ; Kreil, DP; Buerstmayr, H Jahr: 2012
Conference & Workshop proceedings, paper, abstract
Transcriptome Sequencing of Fusarium Challenged Wheat Near Isogenic Lines: A Comparison of Methods
Autoren: Siegwart, G; Ametz, C; Samad-Zamini, M; Steiner, B; Schweiger, W; Buerstmayr, H Jahr: 2012
Conference & Workshop proceedings, paper, abstract
Functional Genomics of UDP-Glucosyltransferases: Heterologous Expression in Yeast to Test for Deoxynivalenol Detoxification Capability of Candidate Genes
Autoren: Schweiger, W;Kovalsky Paris, MP; Wiesenberger, G; Berthiller, F; Lemmens, M; Shin, S; Torres Acosta, JA; Muehlbauer, GJ; Adam, G Jahr: 2012
Conference & Workshop proceedings, paper, abstract
Disruption of intracellular trafficking processes and induced endosomal aggregation by the Fusarium mycotoxin zearalenone.
Autoren: Merz, D; Ranftl, R; Hauser, M-T Jahr: 2012
Conference & Workshop proceedings, paper, abstract
Regulation and function of ARIADNE12 upon UV-B radiation in Arabidopsis.
Autoren: Xie L, Lang-Mladek C, Neubert S, Hauser M-T Jahr: 2012
Conference & Workshop proceedings, paper, abstract
The Fusarium mycotoxin zearalenone (ZON) prevents ethylene biosynthesis in Arabidopsis.
Autoren: Merz, D; Werner, U; Parich, A; Gremmel, C; Hofer, G; Schwanninger, M; Adam, G; Schuhmacher, R; Hauser M-T Jahr: 2012
Conference & Workshop proceedings, paper, abstract
Towards the genetic analysis of growth variations upon UV-B radiation in Arabidopsis accessions.
Autoren: Xie, L; Lang-Mladek, C; Neubert, S; Hauser M-T Jahr: 2012
Conference & Workshop proceedings, paper, abstract
Functional characterization of two clusters of Brachypodium distachyon UDP-glycosyltransferases encoding putative deoxynivalenol detoxification genes.
Autoren: Schweiger, W; Pasquet, JC; Nussbaumer, T; Paris, MP; Wiesenberger, G; Macadré, C; Ametz, C; Berthiller, F; Lemmens, M; Saindrenan, P; Mewes, HW; Mayer, KF; Dufresne, M; Adam, G; Jahr: 2013
Journal articles
Expression QTL mapping for Fusarium Head Blight resistance in Wheat
Autoren: M. Samad Zamini, C. Ametz, E. Sam, G. Siegwart, B. Steiner, M. Lemmens, W. Schweiger, H. Buerstmayr Jahr: 2013
Conference & Workshop proceedings, paper, abstract
Wheat gene network dynamics in response to Fusarium graminearum and functional validation of candidate resistance genes
Autoren: G. Siegwart, C. Ametz, B. Steiner, K. Kugler, T. Nussbaumer, W. Schweiger, K. Mayer, H. Buerstmayr Jahr: 2013
Conference & Workshop proceedings, paper, abstract
Functional characterization of a lipid transfer protein associated with Qfhs.ifa-5A
Autoren: G. Siegwart, J. J. Peter, W. Schweiger, H. Buerstmayr Jahr: 2013
Conference & Workshop proceedings, paper, abstract
Transient silencing of a PR-4 gene decreases type I resistance against Fusarium graminearum
Autoren: J. J. Peter, G. Siegwart, W. Schweiger, B. Steiner and H. Buerstmayr Jahr: 2013
Conference & Workshop proceedings, paper, abstract
Identification and functional characterization of a Brachypodium distachyon UGT involved in detoxification of the Fusarium graminearum mycotoxin, deoxynivalenol.
Autoren: Pasquet, J-C; Schweiger, W; Macadre, C; Morlet, C; Tharreau, V; Saindrenan, P; Adam, G; Dufresne, M; Jahr: 2013
Conference & Workshop proceedings, paper, abstract
In planta inactivation of Fusarium mycotoxins
Autoren: Kovalsky-Paris, MP; Schweiger, W; Wiesenberger, G; Torres-Acosta, JA; Michlmayr, H; Newmister, S; Lemmens, M; Malachova, A; Shin, S; Muehlbauer, G; Weigl-Pollack, T; Fruhmann, P; Mikula, H; Hametner C; Kluger, B; Schuhmacher, R; Krksa, R; Fröhlich, J; Rayment, I; Berthiller, F; Adam G; Jahr: 2013
Conference & Workshop proceedings, paper, abstract
Transcriptomic characterization of two major Fusarium resistance quantitative trait loci (QTLs), Fhb1 and Qfhs.ifa-5A, identifies novel candidate genes.
Autoren: Schweiger, W; Steiner, B; Ametz, C; Siegwart, G; Wiesenberger, G; Berthiller, F; Lemmens, M; Jia, H; Adam, G; Muehlbauer, GJ; Kreil, DP; Buerstmayr, H; Jahr: 2013
Journal articles
New insights into the human metabolism of the Fusarium mycotoxins deoxynivalenol and zearalenone.
Autoren: Warth, B; Sulyok, M; Berthiller, F; Schuhmacher, R; Krska, R; Jahr: 2013
Journal articles
Stable isotopic labelling-assisted untargeted metabolic profiling reveals novel conjugates of the mycotoxin deoxynivalenol in wheat.
Autoren: Kluger, B; Bueschl, C; Lemmens, M; Berthiller, F; Häubl, G; Jaunecker, G; Adam, G; Krska, R; Schuhmacher, R Jahr: 2013
Journal articles
Discovery and Toxicity Assessment of Novel Mycotoxins Produced by US Isolates of Fusarium Graminearum
Autoren: Varga, E; Wiesenberger, G; Hametner, C; Krska, R; Adam, G; Kistler, HC; Berthiller, F Jahr: 2013
Conference & Workshop proceedings, paper, abstract
Genomics-Assisted Breeding for Fusarium Head Blight Resistance in Wheat
Autoren: Buerstmayr Hermann, Buerstmayr Maria, Schweiger Wolfgang, Steiner Barbara Jahr: 2013
Chapter in collected volumes
Breeding wheat for resistance to Fusarium head blight: challenges and possibilities
Autoren: Buerstmayr Hermann, Buerstmayr Maria, Schweiger Wolfgang, Steiner Barbara Jahr: 2013
Conference & Workshop proceedings, paper, abstract
Improving Fusarium resistance in bread and durum wheat
Autoren: Buerstmayr Hermann, Buerstmayr Maria, Schweiger Wolfgang, Steiner Barbara Jahr: 2013
Conference & Workshop proceedings, paper, abstract
Detoxification of the Fusarium graminearum Toxins Deoxynivalenol and Zearalenone by UDP-glucosyltransferases
Autoren: Adam, G; Torres Acosta, J; Kovalsky, P; Schweiger, W; Michlmayr, H; Stückler, R; Wiesenberger, G; Berthiller, F Jahr: 2014
Conference & Workshop proceedings, paper, abstract
Advanced LC-HRMS and GC-MS based methods for metabolomics of Fusarium head blight on wheat
Autoren: Schuhmacher, R; Bueschl, C; Doppler, M; Kluger, B; Neumann, N; Parich, A; Warth, B; Lemmens, M; Adam, G; Krska, R; Jahr: 2014
Conference & Workshop proceedings, paper, abstract
MetExtract II: A Software Suite for Stable Isotope-Assisted Untargeted Metabolomics
Autoren: Bueschl, C; Kluger, B; Neumann, NKN; Doppler, M; Maschietto, V; Thallinger, GG; Meng-Reiterer, J; Krska, R; Schuhmacher, R Jahr: 2017
Journal articles
HIGH-RESOLUTON MAPPING OF WHEAT CHROMOSOME ARM 5AS HARBOURING THE FUSARIUM HEAD BLIGHT RESISTANCE QTL Qfhs.ifa-5A
Autoren: Wagner, C; Buerstmayr, M; Schwarz, P; Steiner, B; Brugger, K; Blazek, L; Barabashi, D; Volante, A; Vale, G; Cativelli, L; Buerstmayr, H Jahr: 2018
Conference & Workshop proceedings, paper, abstract
Final Report of the Special Research Programme SFB F37 Fusarium
Autoren: Adam, G; Jahr: 2018
Forschungsbericht (extern. Auftraggeber)
Plant mechanisms counteracting Fusarium virulence factors, a way to reduce mycotoxin contamination?
Autoren: Adam, G; Svoboda, T; Michlmayr, H; Spörhase, P; Twaruschek, K., Wiesenberger, G; Ceranic, A; Doppler, M; Krska, R; Berthiller, F; Schuhmacher R Jahr: 2019
Conference & Workshop proceedings, paper, abstract
Plant mechanisms counteracting Fusarium virulence factors, a way to reduce mycotoxin contamination?
Autoren: Adam, G; Svoboda, T; Michlmayr, H; Spörhase, P; Twaruschek, K; Wiesenberger, G; Ceranic, A; Doppler, M; Krska, R; Berthiller, F; Schuhmacher; R Jahr: 2019
Conference & Workshop proceedings, paper, abstract
Inhibierung der Glykosylierung des Mykotoxins Deoxynivalenol durch den Fusarium-Metabolit Culmorin
Autoren: Michlmayr, H; Rehak,K; Fruhmann, P; Weber, J; Piatkowska, M; Wiesenberger, G; Berthiller, F; Adam, G Jahr: 2019
Conference & Workshop proceedings, paper, abstract
MetMatch: A Semi-Automated Software Tool for the Comparison and Alignment of LC-HRMS Data from Different Metabolomics Experiments
Autoren: Koch, S; Bueschl, C; Doppler, M; Simader, A; Meng-Reiterer, J; Lemmens, M; Schuhmacher, R Jahr: 2016
Journal articles
Project staff
Gerhard Adam
Ao.Univ.Prof. Dipl.-Ing. Dr.rer.nat. Gerhard Adam
gerhard.adam@boku.ac.at
Tel: +43 1 47654-94480
Project Leader
01.02.2009 - 28.02.2019
Marie-Theres Hauser
Ao.Univ.Prof. Dipl.-Ing. Dr.nat.techn. Marie-Theres Hauser
marie-theres.hauser@boku.ac.at
Tel: +43 1 47654-94240
Sub Projectleader
01.02.2009 - 06.12.2017
Joseph Strauss
Univ.Prof. Mag.rer.nat. Dr.rer.nat. Joseph Strauss
joseph.strauss@boku.ac.at
Tel: +43 1 47654-94420
Sub Projectleader
01.02.2009 - 28.02.2019
Eva Stöger
Univ.Prof. Mag. Dr.rer.nat. Eva Stöger
eva.stoeger@boku.ac.at
Tel: +43 1 47654-94001, 94311
Sub Projectleader
01.02.2009 - 06.12.2017
Andrea Koutnik
Dipl.-HTL-Ing. Andrea Koutnik
andrea.koutnik@boku.ac.at
Tel: +43 1 47654-97306
Project Staff
01.02.2009 - 06.12.2017
Maria Paula Kovalsky Paris
Dipl.-Ing. Maria Paula Kovalsky Paris
Project Staff
01.02.2009 - 06.12.2017
Gerlinde Wiesenberger
Dipl.-Ing. Dr. Gerlinde Wiesenberger
gerlinde.wiesenberger@boku.ac.at
Tel: +43 1 47654-94482
Project Staff
01.02.2009 - 06.12.2017