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Increasing the Diversification Potential of the S-Layer Based Molecular Construction Kit (Strategies for Functional Multilayer Self-Assembly Systems)

Project Leader
Sleytr Uwe B., BOKU Project Leader
Type of Research
Basic Research
Egelseer Eva Maria, Project Staff (bis 30.06.2013)
BOKU Research Units
Institute of Biophysik
Funded by
Air Force Office of Scientific Research, Wilson Blvd. 4015, VA 22203-1954 Arlington, United States (USA)
Biomolecular self-assembly systems are the most challenging modular concepts for generating biomimetic nanomaterials for nanobiotechnological applications. In this context, crystalline bacterial cell surface layers (S-layers), the most commonly observed cell surface structures in prokaryotic organisms (bacteria and archaea), represent a unique self-assembly system optimized during billions of years of biological evaluation. They fulfill key requirements for controlled assembly of supramolecular materials and have the potential for a great variety of applications as patterning element and basic building block for generating more complex supramolecular structures involving all major classes of biological molecules (e.g. proteins, lipids, glycans, and nucleic acids). By using nature´s idea of directed assembly, the planned research project shall extend the molecular construction kit by introducing (1) a novel carbohydrate-based linker with higher flexibility compared to a peptide linker for the nanocontrolled assembly of large (e. g. multimeric) enzymes or other functional molecules, (2) a further lattice symmetry (hexagonal) for production of a S-layer/enzyme fusion protein for the development of a novel biocatalyst, (3) concepts for generating functional multilayer structures by self-assembly processes, and (4) an recombinantly produced archaeal S-layer (Sulfolobus) as essential structural component for the biomimetic approach of an archaeal cell envelope.
microbiology; molecular biology; biological engineering;
archaea; extremozyme; molecular construction kit; self-assembly; S-layer proteins;

Sleytr, U.B., Schuster, B., Egelseer, E.M., Pum, D., Horejs, C.M., Tscheliessnig, R., Ilk, N. (2011): Nanotechnology with S-Layer Proteins as Building Blocks.

In: Horworka, S. (Ed.), Progress in Molecular Biology and Translational Science 103, pp. 277-352; Academic Press, Burlington, US; ISBN 978-0-12-415906-8

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