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Gewählte Publikation:

Woelflingseder, L; Adam, G; Marko, D.
(2020): Suppression of Trichothecene-Mediated Immune Response by the Fusarium Secondary Metabolite Butenolide in Human Colon Epithelial Cells
FRONT NUTR. 2020; 7, 127 FullText FullText_BOKU

Butenolide (BUT, 4-acetamido-4-hydroxy-2-butenoic acid gamma-lactone) is a secondary metabolite produced by severalFusariumspecies and is co-produced with the major trichothecene mycotoxin deoxynivalenol (DON) on cereal grains throughout the world. BUT has low acute toxicity and only very limited occurrence and exposure data are available. The intestinal epithelium represents the first physiological barrier against food contaminants. We aimed to elucidate the intestinal inflammatory response of the human, non-cancer epithelial HCEC-1CT cells to BUT and to characterize potential combinatory interactions with co-occurring trichothecenes, such as DON and NX-3. Using a reporter gene approach, BUT (>= 5 mu M, 20 h) was found to decrease lipopolysaccharide (LPS; 10 ng/mL) induced nuclear factor kappa B (NF-kappa B) activation in a dose-dependent manner, and in combinatory treatments BUT represses trichothecene-induced enhancement of this important inflammatory pathway. Analysis of transcription and secretion levels of NF-kappa B-dependent, pro-inflammatory cytokines, revealed a significant down-regulation of IL-1 beta, IL-6, and TNF-alpha in IL-1 beta-stimulated (25 ng/mL) HCEC-1CT cells after BUT exposure (10 mu M). Trichothecene-induced expression of pro-inflammatory cytokines by the presence of 1 mu M DON or NX-3 was substantially suppressed in the presence of 10 mu M BUT. The emerging mycotoxin BUT has the ability to suppress NF-kappa B-induced intestinal inflammatory response mechanisms and to modulate substantially the immune responsiveness of HCEC-1CT cells after trichothecene treatment. Our results suggest that BUT, present in naturally occurring mixtures ofFusariumfungal metabolites, should be increasingly monitored, and the mechanism of inhibition of NF-kappa B that might affect the pathogenesis or progression of intestinal inflammatory disorders, should be further investigated.
Autor*innen der BOKU Wien:
Adam Gerhard
Wölflingseder Lydia

Find related publications in this database (Keywords)
NF-kappa B
intestinal inflammation
combinatory effects

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