BOKU - Universität für Bodenkultur Wien - Forschungsinformationssystem

Logo BOKU-Forschungsportal

Gewählte Publikation:

Voglauer, R; Chang, MW; Dampier, B; Wieser, M; Baumann, K; Sterovsky, T; Schreiber, M; Katinger, H; Grillari, J.
(2006): SNEV overexpression extends the life span of human endothelial cells.
Exp Cell Res. 2006; 312(6):746-759 FullText FullText_BOKU

In a recent screening for genes downregulated in replicatively senescent human umbilical vein endothelial cells (HUVECs), we have isolated the novel protein SNEV. Since then SNEV has proven as a multifaceted protein playing a role in pre-mRNA splicing, DNA repair, and the ubiquitin/proteosome system. Here, we report that SNEV mRNA decreases in various cell types during replicative senescence, and that it is increased in various immortalized cell lines, as well as in breast tumors, where SNEV transcript levels also correlate with the survival of breast cancer patients. Since these mRNA profiles suggested a role of SNEV in the regulation of cell proliferation, the effect of its overexpression was tested. Thereby, a significant extension of the cellular life span was observed, which was not caused by altered telomerase activity or telomere dynamics but rather by enhanced stress resistance. When SNEV overexpressing cells were treated with bleomycin or bleomycin combined with BSO, inducing DNA damage as well as reactive oxygen species, a significantly lower fraction of apoptotic cells was found in comparison to vector control cells. These data suggest that high levels of SNEV might extend the cellular life span by increasing the resistance to stress or by improving the DNA repair capacity of the cells.
Autor*innen der BOKU Wien:
Baumann Kristin
Grillari Johannes
Grillari Regina
Katinger Hermann
Wieser Matthias
Find related publications in this database (using NML MeSH Indexing)
Adult -
Aged -
Aged, 80 and over -
Breast Neoplasms - diagnosis
Cell Aging - genetics
Cell Proliferation - genetics
Cells, Cultured - genetics
DNA Damage - physiology
DNA Repair - physiology
DNA Repair Enzymes - physiology
Endothelial Cells - cytology
Female - cytology
Gene Expression Regulation - cytology
Humans - cytology
Middle Aged - cytology
Nuclear Proteins - genetics
RNA, Messenger - genetics
Reverse Transcriptase Polymerase Chain Reaction - genetics

Find related publications in this database (Keywords)
endothelial cells
DNA damage
telomerase activity
telomere length

© BOKU Wien Impressum