BOKU - Universität für Bodenkultur Wien - Forschungsinformationssystem
Gewählte Publikation:
Stoiber, H; Frank, I; Spruth, M; Schwendinger, M; Mullauer, B; Windisch, JM; Schneider, R; Katinger, H; Ando, I; Dierich, MP.
(1997):
Inhibition of HIV-1 infection in vitro by monoclonal antibodies to the complement receptor type 3 (CR3): an accessory role for CR3 during virus entry?
Mol Immunol. 1997; 34(12-13):855-863
- Abstract:
- Adhesion molecules are known to contribute to infectivity of HIV-1. Here we tested whether the complement receptor type 3 (CR3, CD11b), an alpha(m) beta(2) integrin, plays an accessory role in the infection process of HIV-1, because ICAM-1, a ligand of CR3, is present on the envelope of HIV-1. In addition, the viral transmembrane protein gp41 shares four regions of homology with the complement component C3, a further CR3 ligand. Infection of PBMCs with HIV-IIIB and primary isolates was partially inhibited by anti-CR3 antibodies. A peptide derived from the complement component C3, covering the CR3-binding site of C3 and sharing strong similarity to the immunosuppressive region of gp41; significantly reduced the HIV-1 titer in infection assays. Recombinant soluble gp41 (rsgp41) and the peptide covering the immunosuppressive domain of gp41 inhibited the resetting of iC3b-coated sheep erythrocytes with U937 via complement receptors (CRs) with an efficiency comparable to monoclonal anti-CR antibodies. In addition, sub-populations of CD4 + and CD8 + T-cells isolated from HIV-infected individuals were found to upregulate CR3 as determined by FACS analysis and on the mRNA level. Since gp41 has been implicated in viral fusion, an interaction of its C3-homology region in gp41 or an interaction of ICAM on the surface of free virus with CRs might contribute to facilitate viral entry. (C) 1998 Elsevier Science Ltd. All rights reserved.
- Autor*innen der BOKU Wien:
- Katinger Hermann
- Windisch Wilhelm Matthias
- Find related publications in this database (using NML MeSH Indexing)
- Anti-HIV Agents - therapeutic use
- Antibodies, Monoclonal - immunology
- CD4-Positive T-Lymphocytes - cytology
- CD8-Positive T-Lymphocytes - cytology
- Cell Separation -
- Flow Cytometry -
- HIV Infections - prevention & control
- HIV-1 - metabolism
- Humans -
- Immunization, Passive -
- Leukocytes, Mononuclear - immunology
- Macrophage-1 Antigen - metabolism
- Membrane Proteins -
- RNA, Messenger - analysis
- Receptors, Complement - genetics
- Rosette Formation -
- Find related publications in this database (Keywords)
- CR3
- adhesion molecule
- HIV
- gp41
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