Gewählte Publikation:

Schähs, M; Strasser, R; Stadlmann, J; Kunert, R; Rademacher, T; Steinkellner, H.
(2007): Production of a monoclonal antibody in plants with a humanized N-glycosylation pattern.
Plant Biotechnol J. 2007; 5(5):657-663 FullText FullText_BOKU

Abstract:

in recent years, plants have become an attractive alternative for the production of recombinant proteins. However, their inability to perform authentic mammalian N-glycosylation may cause limitations for the production of therapeutics. A major concern is the presence of beta 1,2-xylose and core alpha 1,3-fucose residues on complex Winked glycans, as these N-glycan epitopes are immunogenic in mammals. In our attempts towards the humanization of plant N-glycans, we have generated an Arabidopsis thaliana knockout line that synthesizes complex N-glycans lacking immunogenic xylose and fucose epitopes. Here, we report the expression of a monoclonal antibody in these glycan-engineered plants that carry a homogeneous mammalian-like complex N-glycan pattern without beta 1,2-xylose and core alpha 1,3-fucose. Plant and Chinese hamster ovary (CHO)-derived immunoglobulins (IgGs) exhibited no differences in electrophoretic mobility and enzyme-linked immunosorbent specificity assays. Our results demonstrate the feasibility of a knockout strategy for N-glycan engineering of plants towards mammalian-like structures, thus providing a significant improvement in the use of plants as an expression platform.

Autor*innen der BOKU Wien:

Kunert Renate

Stadlmann Johannes

Steinkellner Herta

Strasser Richard

Find related publications in this database (using NML MeSH Indexing)

Animals -

Antibodies, Monoclonal - genetics

Arabidopsis - genetics

CHO Cells - genetics

Cricetinae - genetics

Cricetulus - genetics

Enzyme-Linked Immunosorbent Assay - genetics

Epitopes - chemistry

Gene Deletion - chemistry

Glycosylation - chemistry

Humans - chemistry

Immunoglobulin G - immunology

Plant Leaves - genetics

Plants, Genetically Modified - genetics

Polysaccharides - chemistry

Recombinant Proteins - immunology

Find related publications in this database (Keywords)

antibodies

beta 1,2-xylose and core alpha 1,3-fucose

plant glycan

engineering

recombinant proteins

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