BOKU - Universität für Bodenkultur Wien - Forschungsinformationssystem

Gewählte Publikation:

Gorfer, M; Persak, H; Berger, H; Brynda, S; Bandian, D; Strauss, J; .
(2009): Identification of heavy metal regulated genes from the root associated ascomycete Cadophora finlandica using a genomic microarray.
Mycol Res. 2009; 113(Pt 12):1377-1388 FullText FullText_BOKU

Abstract:
The ascomycete Cadophora finlandica, which can form mycorrhizas with ectomycorrhizal and ericoid hosts, is commonly found in heavy metal polluted soils. To understand the selective advantage of this organism at contaminated sites heavy metal regulated genes from C. finlandica were investigated. For gene identification a strategy based on a genomic microarray was chosen, which allows a rapid, genome-wide screening in genetically poorly characterized organisms. In a preliminary screen eleven plasmids covering eight distinct genomic regions and encoding a total of ten Cd-regulated genes were identified. Northern analyses with RNA from C. finlandica grown in the presence of either Cd, Pb or Zn revealed different transcription patterns in response to the heavy metals present in the growth medium. The Cd-regulated genes are predicted to encode several extracellular proteins with unknown functions, transporters, a centaurin-type regulator of intracellular membrane trafficking, a GNAT-family acetyltransferase and a B-type cyclin. (C) 2009 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
Autor*innen der BOKU Wien:
Strauss Joseph
Find related publications in this database (using NML MeSH Indexing)
Adenosine Triphosphatases/genetics;Adenosine Triphosphatases/metabolism;Adsorption;Ascomycota/drug effects;Ascomycota/genetics*;Ascomycota/metabolism;Biodegradation, Environmental;Cadmium/pharmacology;Computational Biology;Evolution, Molecular;Gene Expression Profiling;Gene Expression Regulation, Fungal*;Genome, Fungal;Industrial Waste;Membrane Transport Proteins/genetics;Membrane Transport Proteins/metabolism;Metals, Heavy/pharmacology*;Multigene Family;Mycorrhizae/genetics;Mycorrhizae/metabolism;Oligonucleotide Array Sequence Analysis;Plant Roots/microbiology*;Sequence Analysis, DNA;Soil Pollutants/pharmacology*;

Find related publications in this database (Keywords)
Cadmium
Cadophora finlandica
Genomic microarray
Heavy metal
Lead
Zinc


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