Gewählte Publikation:

Todorovic, GR; Mentler, A; Popp, M; Hann, S; Kollensperger, G; Rampazzo, N; Blum, WEH.
(2013): Determination of Glyphosate and AMPA in Three Representative Agricultural Austrian Soils with a HPLC-MS/MS Method
SOIL SEDIMENT CONTAM. 2013; 22(3): 332-350. FullText FullText_BOKU

Abstract:

We developed a novel method to quantify adsorbed glyphosate and AMPA in soils based on an extraction utilizing Na-tetraborate, an SPE clean-up step, and subsequent LC-MS detection. Reversed phase-based separation of glyphosate and AMPA was realized after FMOC-derivatization. The quantification involved external calibration and 1,2(13)C, N-15- labeled glyphosate as well as C-13, N-15 labeled AMPA as internal standards. The optimum recovery for extraction was obtained with 40mM Na-tetraborate. The method was applied in three representative soils (Kirchberg, Phyra, and Pixendorf, Austria) where glyphosate was applied by standard agricultural practices. The recovery for glyphosate extracted with 40mM Na-tetraborate buffer was 93.5% (RSD <2%) for glyphosate at Kirchberg-cambisol; 95.7% (RSD < 2%) at Pixendorf- chernozem and 79.1% (RSD <7%) at Phyra-stagnosol. The corresponding values for AMPA were 92.4% (RSD <2%) at Kirchberg, 98.1% (RSD <2%) at Pixendorf and 69.9% (RSD <4%) at Phyra. The limits of detection for glyphosate were 6.8g kg(1)(RSD <10%) at Kirchberg, 4.3g kg(1) (RSD <10%) at Pixendorf, and 46.5g kg(1) (RSD <7%) at Phyra. The limits of detection for AMPA were 26.7g kg(1) (RSD <10%) at Kirchberg, 25.2g kg(1) (RSD <10%) at Pixendorf, and 120.3g kg(1) (RSD <9%) at Phyra. Accordingly, the limits of quantification were 22.7g kg(1)(RSD <5%) for glyphosate, and 88.9g kg(1) (RSD <2%) for AMPA at Kirchberg and respectively 14.4g kg(1) (RSD <6%) and 84g kg(1) (RSD <5%) at Pixendorf and 13.8g kg(1) (RSD <6%) and 87.2g kg(1) (RSD <8%) at Phyra. Both substances in the soils were lower than the LOQ before applying the herbicide Roundup. The influence of higher contents of iron oxides, clay, and acidic pH, resulting in a more pronounced adsorption of glyphosate and AMPA in the soils of Phyra and Kirchberg, is demonstrated.

Autor*innen der BOKU Wien:

Blum Winfried E.H.

Hann Stephan

Köllensperger Gunda

Mentler Axel

Rampazzo Nicola

Rampazzo Todorovic Gorana

Find related publications in this database (Keywords)

Glyphosate

AMPA

chernozem

cambisol

stagnosol

adsorption

HPLC

tandem mass spectrometry

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