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Gewählte Publikation:

Gamerith, C; Gajda, M; Ortner, A; Acero, EH; Guebitz, GM; Ulbricht, M.
(2017): Enzymatic hydrolysis of poly(ethyleneterephthalate) used for and analysed by pore modification of track-etched membranes
NEW BIOTECHNOL. 2017; 39: 42-50. FullText FullText_BOKU

Abstract:
The potential of limited enzymatic poly(ethylene terephthalate) (PET) surface hydrolysis for the modification of track-etched (TE) membranes was investigated. Cutinases 1 and 2 from Thermobifida cellulosilytica as well as a fusion protein of cutinase 1 with the polymer binding module from the polyhydroxyalkanoate depolymerase of Alcaligenes faecalis (Thc_Cut1_PBM) were shown to hydrolyse highly crystalline PET TE membranes with a pore diameter of similar to 120 nm at very narrow size distribution. Furthermore the effects of surface chemistry were investigated by comparison of enzymatic hydrolysis by Thc_Cut1_PBM of "as received" PET TE membranes with two surface functionalized versions towards a "hydrophilic" and a more "hydrophobic" surface. The effects of adsorbed protein and the efficacy of cleaning steps after enzymatic treatment were elucidated by complementary methods for surface analysis and membrane characterization. With the optimized cleaning protocol, all adsorbed protein could be removed from the enzyme-treated membranes and effects of chemical surface functionalization of the PET TE membranes were demonstrated. The highest efficiency of enzymatic surface hydrolysis was observed for the original PET TE membranes, leading to an 0.36% weight loss corresponding to a removal of similar to 3 nm PET from the entire surface of the porous membrane. This correlates very well with the measured increase of barrier pore diameter by 4 nm (a radius reduction? of 2 nm), leading to about a twofold increased water permeability. (C) 2017 Elsevier B.V. All rights reserved.
Autor/innen der BOKU Wien:
Gamerith Caroline
Gübitz Georg
Herrero Acero Enrique
Ortner Andreas
BOKU Gendermonitor:


Find related publications in this database (Keywords)
Enzymatic surface functionalization
Poly(ethylene terephthalate)
Pore modification
Track-etched membrane
Enzyme
Cutinase


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