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Gewählte Publikation:

Vogl, T; Kickenweiz, T; Pitzer, J; Sturmberger, L; Weninger, A; Biggs, BW; Kohler, EM; Baumschlager, A; Fischer, JE; Hyden, P; Wagner, M; Baumann, M; Borth, N; Geier, M; Ajikumar, PK; Glieder, A.
(2018): Engineered bidirectional promoters enable rapid multi-gene co-expression optimization
NAT COMMUN. 2018; 9: FullText FullText_BOKU

Abstract:
Numerous synthetic biology endeavors require well-tuned co-expression of functional components for success. Classically, monodirectional promoters (MDPs) have been used for such applications, but MDPs are limited in terms of multi-gene co-expression capabilities. Consequently, there is a pressing need for new tools with improved flexibility in terms of genetic circuit design, metabolic pathway assembly, and optimization. Here, motivated by naturexxxs use of bidirectional promoters (BDPs) as a solution for efficient gene co-expression, we generate a library of 168 synthetic BDPs in the yeast Komagataella phaffii (syn. Pichia pastoris), leveraging naturally occurring BDPs as a parts repository. This library of synthetic BDPs allows for rapid screening of diverse expression profiles and ratios to optimize gene co-expression, including for metabolic pathways (taxadiene, beta-carotene). The modular design strategies applied for creating the BDP library could be relevant in other eukaryotic hosts, enabling a myriad of metabolic engineering and synthetic biology applications.
Autor/innen der BOKU Wien:
Baumann Martina
Borth Nicole
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