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Gewählte Publikation:

Wimberger, T; Peham, JR; Ehmoser, EK; Wassermann, KJ.
(2019): Controllable cell manipulation in a microfluidic pipette-tip design using capacitive coupling of electric fields
LAB CHIP. 2019; 19(23): 3997-4006. FullText FullText_BOKU

Abstract:
Systems designed toward cell manipulation by electric fields are inherently challenged by energy dissipation along the electrode-electrolyte interface. A promising remedy is the introduction of high-k electrode passivation, enabling efficient capacitive coupling of electric fields into biological samples. We present the implementation of this strategy in a reusable pipette tip design featuring a 10 mu l chamber volume for life science applications. Prototype validation and comparison to conductive gold-coated electrodes reveal a consistent and controllable biological effect that significantly increases the reproducibility of lysis events. The system provides precise descriptions of HEK-293 lysis dependency to variables such as field strength, frequency, and conductivity. Over 80% of cells were reversibly electroporated with minimal electrical lysis over a broad range of field settings. Successful transfection requires exponential decay pulses and showcases how modulating capacitive coupling can advance our understanding of fundamental mechanics in the field of electroporation.
Autor/innen der BOKU Wien:
Ehmoser Eva-Kathrin
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