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Gewählte Publikation:

Gauthier, T; Duarte-Hospital, C; Vignard, J; Boutet-Robinet, E; Sulyok, M; Snini, SP; Alassane-Kpembi, I; Lippi, Y; Puel, S; Oswald, IP; Puel, O.
(2020): Versicolorin A, a precursor in aflatoxins biosynthesis, is a food contaminant toxic for human intestinal cells
ENVIRON INT. 2020; 137, UNSP 105568 FullText FullText_BOKU

Abstract:
Aflatoxin B-1 (AFB(1)) is the most potent carcinogen among mycotoxins. Its biosynthesis involves the formation of versicolorin A (VerA), whose chemical structure shares many features with AFB1. Our data revealed significant levels of VerA in foodstuff from Central Asia and Africa. Given this emerging food risk, it was of prime interest to compare the toxic effects of the two mycotoxins against cells originating from the intestinal tract. We used human colon cell lines (Caco-2, HCT116) to investigate the cytotoxic process induced by the two mycotoxins. Contrary to AFB1, a low dose of VerA (1 mu M) disturbed the expression level of thousands of genes (18 002 genes). We show that the cytotoxic effects of low doses of VerA (1-20 mu M) were stronger than the same low doses of AFB1 in both Caco-2 and HCT116 cell lines. In Caco-2 cells, VerA induced DNA strand breaks that led to apoptosis and reduced DNA replication of dividing cells, consequently inhibiting cell proliferation. Although VerA was able to induce the p53 signaling pathway in p53 wild-type HCT116 cells, its toxicity process did not mainly rely on p53 expression since similar cytotoxic effects were also observed in HCT116 cells that do not express p53. In conclusion, this study provides evidence of the risk of food contamination by VerA and shed light on its toxicological effect on human colon cells.
Autor/innen der BOKU Wien:
Sulyok Michael
BOKU Gendermonitor:


Find related publications in this database (Keywords)
Mycotoxins
Versicolorin A
Aflatoxin B1
Human colon cells
Genotoxicity
Cytotoxicity


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