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Gewählte Publikation:

Yang, S; Diem, M; Liu, JDH; Wesseling, S; Vervoort, J; Oostenbrink, C; Rietjens, IMCM.
(2020): Cellular levels and molecular dynamics simulations of estragole DNA adducts point at inefficient repair resulting from limited distortion of the double-stranded DNA helix
ARCH TOXICOL. 2020; 94(4): 1349-1365. FullText FullText_BOKU

Estragole, naturally occurring in a variety of herbs and spices, can form DNA adducts after bioactivation. Estragole DNA adduct formation and repair was studied in in vitro liver cell models, and a molecular dynamics simulation was used to investigate the conformation dependent (in)efficiency of N-2-(trans-isoestragol-3 '-yl)-2 '-deoxyguanosine (E-3 '-N-2-dG) DNA adduct repair. HepG2, HepaRG cells, primary rat hepatocytes and CHO cells (including CHO wild-type and three NER-deficient mutants) were exposed to 50 mu M estragole or 1 '-hydroxyestragole and DNA adduct formation was quantified by LC-MS immediately following exposure and after a period of repair. Results obtained from CHO cell lines indicated that NER plays a role in repair of E-3 '-N-2-dG adducts, however, with limited efficiency since in the CHO wt cells 80% DNA adducts remained upon 24 h repair. Inefficiency of DNA repair was also found in HepaRG cells and primary rat hepatocytes. Changes in DNA structure resulting from E-3 '-N-2-dG adduct formation were investigated by molecular dynamics simulations. Results from molecular dynamics simulations revealed that conformational changes in double-stranded DNA by E-3 '-N-2-dG adduct formation are small, providing a possible explanation for the restrained repair, which may require larger distortions in the DNA structure. NER-mediated enzymatic repair of E-3 '-N-2-dG DNA adducts upon exposure to estragole will be limited, providing opportunities for accumulation of damage upon repeated daily exposure. The inability of this enzymatic repair is likely due to a limited distortion of the DNA double-stranded helix resulting in inefficient activation of nucleotide excision repair.
Autor/innen der BOKU Wien:
Diem Matthias
Oostenbrink Chris
BOKU Gendermonitor:

Find related publications in this database (Keywords)
DNA adduct
DNA repair efficiency
Molecular modeling and simulation

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