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Gewählte Publikation:

M?csai, R; Figl, R; S?tzl, L; Fluch, S; Altmann, F.
(2020): A first view on the unsuspected intragenus diversity of N-glycans inChlorellamicroalgae
PLANT J. 2020; 103(1): 184-196. FullText FullText_BOKU

Abstract:
Chlorellamicroalgae are increasingly used for various purposes such as fatty acid production, wastewater processing, or as health-promoting food supplements. A mass spectrometry-based survey of N-glycan structures of strain collection specimens and 80 commercialChlorellaproducts revealed a hitherto unseen intragenus diversity of N-glycan structures. Differing numbers of methyl groups, pentoses, deoxyhexoses, andN-acetylglucosamine culminated inc.100 different glycan masses. Thirteen clearly discernible glycan-type groups were identified. Unexpected features included the occurrence of arabinose, of different and rare types of monosaccharide methylation (e.g. 4-O-methyl-N-acetylglucosamine), and substitution of the secondN-acetylglucosamine. Analysis of barcode ITS1-5.8S-ITS2 rDNA sequences established a phylogenetic tree that essentially went hand in hand with the grouping obtained by glycan patterns. This brief prelude to microalgal N-glycans revealed a fabulous wealth of undescribed structural features that finely differentiatedChlorella-like microalgae, which are notoriously poor in morphological attributes. In light of the almost identical N-glycan structural features that exist within vertebrates or land plants, the herein discovered diversity is astonishing and argues for a selection pressure only explicable by a fundamental functional role of these glycans.
Autor/innen der BOKU Wien:
Altmann Friedrich
Figl Rudolf
Mocsai Reka Tünde
Sützl Leander
BOKU Gendermonitor:


Find related publications in this database (Keywords)
microalgae
N-glycans
Chlorella vulgaris
Chlorella sorokiniana
Chlorella pyrenoidosa
arabinose
methylated sugar


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