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Gewählte Publikation:

Solin, K; Beaumont, M; Rosenfeldt, S; Orelma, H; Borghei, M; Bacher, M; Opietnik, M; Rojas, OJ; .
(2020): Self-Assembly of Soft Cellulose Nanospheres into Colloidal Gel Layers with Enhanced Protein Adsorption Capability for Next-Generation Immunoassays.
Small. 2020; 16(50):e2004702 FullText FullText_BOKU

Abstract:
Soft cationic core/shell cellulose nanospheres can deform and interpenetrate allowing their self-assembly into densely packed colloidal nanogel layers. Taking advantage of their water-swelling capacity and molecular accessibility, the nanogels are proposed as a new and promising type of coating material to immobilize bioactive molecules on thin films and paper. The specific and nonspecific interactions between the cellulosic nanogel and human immunoglobulin G as well as bovine serum albumin (BSA) are investigated. Confocal microscopy, electroacoustic microgravimetry, and surface plasmon resonance are used to access information about the adsorption behavior and viscoelastic properties of self-assembled nanogels. A significant BSA adsorption capacity on nanogel layers (17 mg m(-2)) is measured, 300% higher compared to typical polymer coatings. This high protein affinity further confirms the promise of the introduced colloidal gel layer, in increasing sensitivity and advancing a new generation of substrates for a variety of applications, including immunoassays, as demonstrated in this work.
Autor/innen der BOKU Wien:
Bacher Markus
Beaumont Marco
BOKU Gendermonitor:


Find related publications in this database (Keywords)
amorphous nanocellulose
cellulose II nanogel
colloids
core
shell nanoparticles
immunosensors
protein adsorption


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