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Gewählte Publikation:

Tegl, G; Thallinger, B; Beer, B; Sygmund, C; Ludwig, R; Rollett, A; Nyanhongo, GS; Guebitz, GM.
(2016): Antimicrobial Cellobiose Dehydrogenase-Chitosan Particles
ACS APPL MATER INTER. 2016; 8(1): 967-973. FullText FullText_BOKU

Abstract:
Increasing prevalence of chronic wounds and microbial infection constitute a severe health challenge. The situation is further complicated by emerging multidrug resistance making the treatment of infections increasingly difficult. Here, a novel antimicrobial system based on in situ release of hydrogen peroxide (H2O2) by cellobiose dehydrogenase (CDH) immobilized on chitosan (CTS) particles is described. Covalent immobilization using carbodiimide coupling lead to a higher amount of protein immobilized on CTS (104 mu g CDH/mg CTS) when compared to noncovalent immobilization, which, however, showed highest recovery of CDH activity (0.01 U/mg CTS). The CDH-CTS in situ generated H2O2 completely inhibited growth of Escherichia coli and Staphylococcus aureus over a period of 24 h. This resilient antimicrobial system represents a novel strategy for preventing infection with potential application in counteracting microbial colonization of chronic wounds.
Autor/innen der BOKU Wien:
Beer Bianca
Gübitz Georg
Herrero-Rollett Alexandra
Ludwig Roland
Nyanhongo Gibson Stephen
Sygmund Christoph
Tegl Gregor Franz
Thallinger Barbara
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Find related publications in this database (Keywords)
cellobiose dehydrogenase
chronic wounds
infection
immobilization
enzyme
hydrogen peroxide


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