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Gewählte Publikation:

Patel, I; Kracher, D; Ma, S; Garajova, S; Haon, M; Faulds, CB; Berrin, JG; Ludwig, R; Record, E.
(2016): Salt-responsive lytic polysaccharide monooxygenases from the mangrove fungus Pestalotiopsis sp NCi6
BIOTECHNOL BIOFUELS. 2016; 9: FullText FullText_BOKU

Abstract:
Background: Lytic polysaccharide monooxygenases (LPMOs) belong to the "auxiliary activities (AA)" enzyme class of the CAZy database. They are known to strongly improve the saccharification process and boost soluble sugar yields from lignocellulosic biomass, which is a key step in the efficient production of sustainable economic biofuels. To date, most LPMOs have been characterized from terrestrial fungi, but novel fungal LPMOs isolated from more extreme environments such as an estuary mangrove ecosystem could offer enzymes with unique properties in terms of salt tolerance and higher stability under harsh condition. Results: Two LPMOs secreted by the mangrove-associated fungus Pestalotiopsis sp. NCi6 (PsLPMOA and PsLPMOB) were expressed in the yeast Pichia pastoris and produced in a bioreactor with >85 mg L-1 for PsLPMOA and >260 mg L-1 for PsLPMOB. Structure-guided homology modeling of the PsLPMOs showed a high abundance of negative surface charges, enabling enhanced protein stability and activity in the presence of sea salt. Both PsLPMOs were activated by a cellobiose dehydrogenase (CDH) from Neurospora crassa, with an apparent optimum of interaction at pH 5.5. Investigation into their regioselective mode of action revealed that PsLPMOA released C1- and C4-oxidized cello-oligosaccharide products, while PsLPMOB released only C4-oxidized products. PsLPMOA was found to cleave polymeric cellulose in the presence of up to 6 % sea salt, which emphasizes the use of sea water in the industrial saccharification process with improved ecological footprints. Conclusions: Two new LPMOs from the mangrove fungus Pestalotiopsis sp. NCi6 were found to be fully reactive against cellulose. The combined hydrolytic activities of these salt-responsive LPMOs could therefore facilitate the saccharification process using sea water as a reaction medium for large-scale biorefineries.
Autor/innen der BOKU Wien:
Kracher Daniel
Ludwig Roland
Ma Su
BOKU Gendermonitor:


Find related publications in this database (Keywords)
AA9
LPMO
Cellobiose dehydrogenase
Cellulose
Biorefinery


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