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Gewählte Publikation:

Warth, B; Del Favero, G; Wiesenberger, G; Puntscher, H; Woelflingseder, L; Fruhmann, P; Sarkanj, B; Krska, R; Schuhmacher, R; Adam, G; Marko, D.
(2016): Identification of a novel human deoxynivalenol metabolite enhancing proliferation of intestinal and urinary bladder cells
SCI REP-UK. 2016; 6: FullText FullText_BOKU

The mycotoxin deoxynivalenol (DON) is an abundant contaminant of cereal based food and a severe issue for global food safety. We report the discovery of DON-3-sulfate as a novel human metabolite and potential new biomarker of DON exposure. The conjugate was detectable in 70% of urine samples obtained from pregnant women in Croatia. For the measurement of urinary metabolites, a highly sensitive and selective LC-MS/MS method was developed and validated. The method was also used to investigate samples from a duplicate diet survey for studying the toxicokinetics of DON-3-sulfate. To get a preliminary insight into the biological relevance of the newly discovered DON-sulfates, in vitro experiments were performed. In contrast to DON, sulfate conjugates lacked potency to suppress protein translation. However, surprisingly we found that DON-sulfates enhanced proliferation of human HT-29 colon carcinoma cells, primary human colon epithelial cells (HCEC-1CT) and, to some extent, also T24 bladder cancer cells. A proliferative stimulus, especially in tumorigenic cells raises concern on the potential impact of DON-sulfates on consumer health. Thus, a further characterization of their toxicological relevance should be of high priority.
Autor*innen der BOKU Wien:
Adam Gerhard
Krska Rudolf
Schuhmacher Rainer
Warth Benedikt
Wiesenberger Gerlinde
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