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Gewählte Publikation:

Oehlknecht, C; Tegl, G; Beer, B; Sygmund, C; Ludwig, R; Guebitz, GM.
(2017): Cellobiose dehydrogenase and chitosan-based lysozyme responsive materials for antimicrobial wound treatment
BIOTECHNOL BIOENG, 114, 416-422; ISSN 0006-3592 FullText FullText_BOKU

Abstract:
The treatment of wound infection still constitutes a major threat in health care due to the increasing number of bacterial resistances and the difficulty of timely infection detection. Here, we present a smart antimicrobial system that is activated in case of infection based on elevated lysozyme activities. N-acetyl chitosan (degree of N-acetylation: 40%) was synthesized and hydrolysis by lysozyme in artificial wound fluid (AWF) was demonstrated. This resulted in the formation of N-acetylated chito oligosaccharides (COS) with a degree of polymerization of 2-5 units. The COS were shown to serve as substrate for cellobiose dehydrogenase (CDH) leading to the production of 1mM antimicrobial hydrogen peroxide (H2O2) after 24h incubation at 37 degrees C in AWF. Growth inhibition was seen upon incubation of Escherichia coli and Staphylococcus aureus with this chitosan-CDH system over 8h. This approach represents the first self-regulating system for the infection responsive inhibition of bacterial growth in response to lysozyme as infection biomarker. Biotechnol. Bioeng. 2017;114: 416-422. (c) 2016 Wiley Periodicals, Inc.
Autor/innen der BOKU Wien:
Beer Bianca
Gübitz Georg
Ludwig Roland
Öhlknecht Christoph
Sygmund Christoph
Tegl Gregor Franz
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Find related publications in this database (Keywords)
infection prevention
infection detection
N-acetyl chitosan
enzyme responsive
diagnostics


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