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Gewählte Publikation:

Binder, SB; Schwartz-Zimmermann, HE; Varga, E; Bichl, G; Michlmayr, H; Adam, G; Berthiller, F; .
(2017): Metabolism of Zearalenone and Its Major Modified Forms in Pigs.
Toxins (Basel). 2017; 9(2): FullText FullText_BOKU

Abstract:
The Fusarium mycotoxin zearalenone (ZEN) can be conjugated with polar molecules, like sugars or sulfates, by plants and fungi. To date, the fate of these modified forms of ZEN has not yet been elucidated in animals. In order to investigate whether ZEN conjugates contribute to the total ZEN exposure of an individual, ZEN (10 mu g/ kg b.w.) and equimolar amounts of two of its plant metabolites (ZEN-14-O-beta-glucoside, ZEN-16-O-beta-glucoside) and of one fungal metabolite (ZEN-14-sulfate) were orally administered to four pigs as a single bolus using a repeated measures design. The concentrations of ZEN, its modified forms and its mammalian metabolites ZEN-14-glucuronide, alpha-zearalenol (alpha-ZEL) and alpha-ZEL-14-glucuronide in excreta were analyzed by high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/ MS) based methods. The biological recovery of ZEN in urine was 26% +/- 10%, the total biological recovery in excreta was 40% +/- 8%. Intact ZEN-14-sulfate, ZEN-14-O-beta -glucoside and ZEN-16-O-beta-glucoside were neither detected in urine nor in feces. After ZEN-14-sulfate application, 19% +/- 5% of the administered dose was recovered in urine. In feces, no ZEN metabolites were detected. The total biological recoveries of ZEN-14-O-beta-glucoside and ZEN-16-O-beta-glucoside in the form of their metabolites in urine were 19% +/- 11% and 13% +/- 7%, respectively. The total biological recoveries in urine and feces amounted to 48% +/- 7% and 34 +/- 3%. An explanation for the low biological recoveries could be extensive metabolization by intestinal bacteria to yet unknown metabolites. In summary, ZEN-14-sulfate, ZEN-14-O-beta-glucoside, and ZEN-16-O-beta-glucoside were completely hydrolyzed in the gastrointestinal tract of swine, thus contributing to the overall toxicity of ZEN.
Autor*innen der BOKU Wien:
Adam Gerhard
Berthiller Franz
Michlmayr Herbert
Schwartz-Zimmermann Heidi Elisabeth
Varga Elisabeth
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
Administration, Oral;Animals;Chromatography, High Pressure Liquid;Feces/chemistry;Food Microbiology*;Glucosides/metabolism;Glucuronides/metabolism;Hydrolysis;Intestinal Absorption;Intestinal Elimination;Male;Metabolic Detoxication, Phase II;Renal Elimination;Sus scrofa;Tandem Mass Spectrometry;Zearalenone/administration & dosage;Zearalenone/analogs & derivatives;Zearalenone/metabolism*;Zearalenone/toxicity;Zearalenone/urine;Zeranol/analogs & derivatives;Zeranol/metabolism;

Find related publications in this database (Keywords)
mycotoxins
masked mycotoxins
plant metabolites
bioavailability
cleavage
enzymatic hydrolysis
gastrointestinal tract
high performance liquid chromatography tandem mass spectrometry


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