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Gewählte Publikation:

Tavahodi, M; Ortiz, R; Schulz, C; Ekhtiari, A; Ludwig, R; Haghighi, B; Gorton, L.
(2017): Direct Electron Transfer of Cellobiose Dehydrogenase on Positively Charged Polyethyleneimine Gold Nanoparticles
CHEMPLUSCHEM. 2017; 82(4): 546-552. FullText FullText_BOKU

Abstract:
Efficient conjugation between biomolecules and electrode materials is one of the main challenges in the field of biosensors. Cellobiose dehydrogenase (CDH) is a monomeric enzyme, which consists of two separate domains: one catalytic dehydrogenase domain (DHCDH) carrying strongly bound flavin adenine dinucleotide (FAD) in the active site and a cytochrome domain (CYTCDH) carrying a b-type heme connected by a flexible linker region. Herein, we report on the development of a lactose biosensor, based on direct electron transfer (DET) from CDH from Phanerochaete sordida (PsCDH) electrostatically attached onto polyethyleneimine-stabilized gold nanoparticles (PEI@AuNPs) used to cover a conventional polycrystalline solid gold disk electrode. PEI@AuNPs were synthesized in aqueous solution using PEI as reducing agent for Au-III and as stabilizer for the nanoparticles. The heterogeneous electron-transfer (ET) rate (k(s)) for the redox reaction of immobilized PsCDH at the modified electrodes was calculated based on the Laviron theory and was found to be (39.6 +/- 2.5) s(-1). The proposed lactose biosensor exhibits good long term stability as well as high and reproducible sensitivity to lactose with a response time less than 5 s and a linear range from 1 to 100 mu m.
Autor/innen der BOKU Wien:
Ludwig Roland
BOKU Gendermonitor:


Find related publications in this database (Keywords)
biosensors
electron transfer
enzymes
gold
nanoparticles


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