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Gewählte Publikation:

Siepenkoetter, T; Salaj-Kosla, U; Xiao, XX; Conghaile, PO; Pita, M; Ludwig, R; Magner, E.
(2017): Immobilization of Redox Enzymes on Nanoporous Gold Electrodes: Applications in Biofuel Cells
CHEMPLUSCHEM. 2017; 82(4): 553-560. FullText FullText_BOKU

Abstract:
Nanoporous gold (NPG) electrodes were prepared by dealloying sputtered gold: silver alloys. Electrodes of different thicknesses and pore sizes areas were prepared by varying the temperature and duration of the dealloying procedure; these were then used as supports for FAD-dependent glucose dehydrogenase (GDH) (Glomorella cingulata) and bilirubin oxidase (BOx) (Myrothecium verrucaria). Glucose dehydrogenase was immobilized by drop-casting a solution of the enzyme with an osmium redox polymer together with a crosslinked polymer, whereas bilirubin oxidase was attached covalently through carbodiimide coupling to a diazonium-modified NPG electrode. The stability of the bilirubin-oxidase-modified NPG electrode was significantly improved in comparison with that of a planar gold electrode. Enzyme fuel cells were also prepared; the optimal response was obtained with a BOx-modified NPG cathode (500 nm thickness) and a GDH-modified anode (300 nm), which generated power densities of 17.5 and 7.0 mu W cm(-2) in phosphate-buffered saline and artificial serum, respectively.
Autor/innen der BOKU Wien:
Ludwig Roland
BOKU Gendermonitor:


Find related publications in this database (Keywords)
biofuel cells
electrochemistry
gold
mesoporous materials
nanoelectrodes


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