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Gewählte Publikation:

Linke, RB; Kebede, G; Mushi, D; Lakew, A; Hayes, DS; Graf, W; Farnleitner, AH.
(2021): Assessing the faecal source sensitivity and specificity of ruminant and human genetic microbial source tracking markers in the central Ethiopian highlands
LETT APPL MICROBIOL. 2021; 72(4): 458-466. FullText FullText_BOKU

Abstract:
This study tested genetic microbial source tracking (MST) methods for identifying ruminant- (BacR) and human-associated (HF183/BacR287, BacHum) bacterial faecal contaminants in Ethiopia in a newly created regional faecal sample bank (n = 173). BacR performed well, and its marker abundance was high (100% sensitivity (Sens), 95% specificity (Spec), median log(10) 8 center dot 1 marker equivalents (ME) g(-1) ruminant faeces). Human-associated markers tested were less abundant in individual human samples (median: log(10) 5 center dot 4 and 4 center dot 2 (ME + 1) g(-1)) and were not continuously detected (81% Sens, 91% Spec for BacHum; 77% Sens, 91% Spec for HF183/BacR287). Furthermore, the pig-associated Pig2Bac assay was included and performed excellent (100% Sens, 100% Spec). To evaluate the presence of MST targets in the soil microbiome, representative soil samples were tested during a whole seasonal cycle (n = 60). Only BacR could be detected, but was limited to the dry season and to sites of higher anthropogenic influence (log(10) 3 center dot 0 to 4 center dot 9 (ME + 1) g(-1) soil). In conclusion, the large differences in marker abundances between target and non-target faecal samples (median distances between distributions >= log(10) 3 to >= log(10) 7) and their absence in pristine soil indicate that all tested assays are suitable candidates for diverse MST applications in the Ethiopian area.
Autor*innen der BOKU Wien:
Graf Wolfram
Hayes Daniel S.

Find related publications in this database (Keywords)
assay evaluation
Ethiopia
faeces
human
marker
microbial source tracking
ruminant
soil


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