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Gewählte Publikation:

Ceranic, A; Svoboda, T; Berthiller, F; Sulyok, M; Samson, JM; Guldener, U; Schuhmacher, R; Adam, G.
(2021): Identification and Functional Characterization of the Gene Cluster Responsible for Fusaproliferin Biosynthesis in Fusarium proliferatum
TOXINS. 2021; 13(7), 468 FullText FullText_BOKU

Abstract:
The emerging mycotoxin fusaproliferin is produced by Fusarium proliferatum and other related Fusarium species. Several fungi from other taxonomic groups were also reported to produce fusaproliferin or the deacetylated derivative, known as siccanol or terpestacin. Here, we describe the identification and functional characterization of the Fusarium proliferatum genes encoding the fusaproliferin biosynthetic enzymes: a terpenoid synthase, two cytochrome P450s, a FAD-oxidase and an acetyltransferase. With the exception of one gene encoding a CYP450 (FUP2, FPRN_05484), knock-out mutants of the candidate genes could be generated, and the production of fusaproliferin and intermediates was tested by LC-MS/MS. Inactivation of the FUP1 (FPRN_05485) terpenoid synthase gene led to complete loss of fusaproliferin production. Disruption of a putative FAD-oxidase (FUP4, FPRN_05486) did not only affect oxidation of preterpestacin III to terpestacin, but also of new side products (11-oxo-preterpstacin and terpestacin aldehyde). In the knock-out strains lacking the predicted acetyltransferase (FUP5, FPRN_05487) fusaproliferin was no longer formed, but terpestacin was found at elevated levels. A model for the biosynthesis of fusaproliferin and of novel derivatives found in mutants is presented.
Autor*innen der BOKU Wien:
Adam Gerhard
Berthiller Franz
Ceranic Asja
Samson Jonathan Matthew
Schuhmacher Rainer
Sulyok Michael
Svoboda Thomas
BOKU Gendermonitor:


Find related publications in this database (Keywords)
fusaproliferin
terpestacin
preterpestacin
emerging mycotoxins
knock-out
terpenoid synthase
cytochrome P450 oxidoreductase
FAD-oxidase
acetyltransferase


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