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Gewählte Publikation:

Poppenberger, B; Berthiller, F; Lucyshyn, D; Sieberer, T; Schuhmacher, R; Krska, R; Kuchler, K; Glössl, J; Luschnig, C; Adam, G.
(2003): Detoxification of the Fusarium mycotoxin deoxynivalenol by a UDP-glucosyltransferase from Arabidopsis thaliana.
J Biol Chem. 2003; 278(48):47905-47914 FullText FullText_BOKU

Plant pathogenic fungi of the genus Fusarium cause agriculturally important diseases of small grain cereals and maize. Trichothecenes are a class of mycotoxins produced by different Fusarium species that inhibit eukaryotic protein biosynthesis and presumably interfere with the expression of genes induced during the defense response of the plants. One of its members, deoxynivalenol, most likely acts as a virulence factor during fungal pathogenesis and frequently accumulates in grain to levels posing a threat to human and animal health. We report the isolation and characterization of a gene from Arabidopsis thaliana encoding a UDP-glycosyltransferase that is able to detoxify deoxynivalenol. The enzyme, previously assigned the identifier UGT73C5, catalyzes the transfer of glucose from UDP-glucose to the hydroxyl group at carbon 3 of deoxynivalenol. Using a wheat germ extract-coupled transcription/ translation system we have shown that this enzymatic reaction inactivates the mycotoxin. This deoxynivalenol-glucosyltransferase (DOGT1) was also found to detoxify the acetylated derivative 15-acetyl-deoxynivalenol, whereas no protective activity was observed against the structurally similar nivalenol. Expression of the glucosyltransferase is developmentally regulated and induced by deoxynivalenol as well as salicylic acid, ethylene, and jasmonic acid. Constitutive overexpression in Arabidopsis leads to enhanced tolerance against deoxynivalenol.
Autor*innen der BOKU Wien:
Adam Gerhard
Berthiller Franz
Glößl Josef
Krska Rudolf
Lucyshyn Doris
Luschnig Christian
Schuhmacher Rainer
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Find related publications in this database (using NML MeSH Indexing)
Amino Acid Sequence -
Arabidopsis - enzymology
Arabidopsis Proteins - enzymology
Carbon - chemistry
Cloning, Molecular - chemistry
DNA, Complementary - metabolism
Escherichia coli - metabolism
Fusarium - metabolism
Gene Library - metabolism
Genetic Vectors - metabolism
Glucosyltransferases - chemistry
Glutathione Transferase - metabolism
Glycosylation - metabolism
Mass Spectrometry - metabolism
Models, Chemical - metabolism
Molecular Sequence Data - metabolism
Mycotoxins - metabolism
Phenotype - metabolism
Promoter Regions, Genetic - metabolism
Protein Conformation - metabolism
RNA, Messenger - metabolism
Reverse Transcriptase Polymerase Chain Reaction - metabolism
Ribosomes - metabolism
Sequence Homology, Amino Acid - metabolism
Transgenes - metabolism
Trichothecenes - metabolism
Uridine Diphosphate Glucose - metabolism

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