BOKU - Universität für Bodenkultur Wien - Forschungsinformationssystem

Logo BOKU-Forschungsportal

Gewählte Publikation:

Ludwig, R; Salamon, A; Varga, J; Zámocky, M; Peterbauer, CK; Kulbe, KD; Haltrich, D; .
(2004): Characterisation of cellobiose dehydrogenases from the white-rot fungi Trametes pubescens and Trametes villosa.
Appl Microbiol Biotechnol. 2004; 64(2):213-222 FullText FullText_BOKU

Abstract:
Cellobiose dehydrogenase (CDH) is an extracellular haemoflavoenzyme that is produced by a number of wood-degrading and phytopathogenic fungi and it has a proposed role in the early events of lignocellulose degradation and wood colonisation. In the presence of a suitable electron acceptor, e.g. 2,6-dichloro-indophenol, cytochrome c, or metal ions, CDH oxidises cellobiose to cellobionolactone. When screening 11 different Trametes spp. for the formation of CDH activity, all the strains investigated were found to secrete significant amounts of CDH when cultivated on a cellulose-containing medium. Amongst others, Trametes pubescens and Trametes villosa were identified as excellent, not-yet-described, producer strains of this enzyme activity that has various potential applications in biotechnology. CDH from both strains was purified to apparent homogeneity and subsequently characterised. Both monomeric enzymes have a molecular mass of approximately 90 kDa (gel filtration) and a pI value of 4.2-4.4. The best substrates are cellobiose and cellooligosaccharides; additionally, lactose, thiocellobiose, and xylobiose are efficiently oxidised. Glucose and maltose are poor substrates. The preferred substrate is cellobiose with a K-m value of 0.21 mM and a k(cat) value of 22 s(-1) for CDH from T. pubescens; the corresponding values for the T. villosa enzyme are 0.21 mM and 24 s(-1), respectively. Both enzymes showed very high activity with one-electron acceptors such as ferricenium, ferricyanide, or the azino-bis-(3-ethyl-benzthiazolin-6-sulfonic acid) cation radical.
Autor/innen der BOKU Wien:
Haltrich Dietmar
Kulbe Klaus Dieter
Ludwig Roland
Peterbauer Clemens Karl
Zamocky Marcel
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
2,6-Dichloroindophenol/metabolism;Bioreactors;Carbohydrate Dehydrogenases/isolation & purification*;Carbohydrate Dehydrogenases/metabolism*;Cellobiose/analogs & derivatives*;Cellobiose/metabolism;Cellulose/metabolism;Chromatography, Gel;Chromatography, Ion Exchange;Cytochromes c/chemistry;Disaccharides/metabolism;Electron Transport;Ferricyanides/metabolism;Ferrous Compounds/metabolism;Glucose/metabolism;Isoelectric Point;Lactose/metabolism;Maltose/metabolism;Molecular Weight;Oxidation-Reduction;Polyporales/enzymology*;Polyporales/growth & development;Polyporales/metabolism;Substrate Specificity;Thioglycosides/metabolism;



© BOKU Wien Impressum