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Gewählte Publikation:

Ludwig, R., Ozga, M., Zámocky, M., Peterbauer, C., Kulbe, K. D., and Haltrich, D..
(2004): Continuous enzymatic regeneration of electron acceptors used by flavoenzymes: Cellobiose dehydrogenase-catalyzed production of lactobionic acid as an example
Biocatalysis Biotrans., 22, 97–104 FullText FullText_BOKU

Abstract:
An efficient enzymatic bioprocess is described in which lactose, an abundant renewable resource produced by the dairy industry, is completely and efficiently converted with a specific productivity of up to 32 g (kU h)(-1) into lactobionic acid, without the formation of any by-products. The key biocatalyst of this new process is the fungal enzyme cellobiose dehydrogenase which oxidizes several beta-1,4-linked disaccharides including lactose specifically at position C-1 of the reducing sugar moiety to the corresponding lactones. The electron acceptor employed in this reaction is continuously regenerated with the help of laccase, a H2O-producing, copper-containing oxidase, and therefore has to be added in low, catalytic amounts only. Redox mediators that were successfully employed in this novel process and hence are compatible with the laccase regeneration system include benzoquinone, ABTS, ferricyanide, or ferrocene, amongst others. Factors affecting operational stability of the biocatalysts employed in this process include the redox mediator used, the temperature, and importantly the volumetric gas flow necessary for maintaining the dissolved oxygen tension. Lactobionic acid is a mild and sweet tasting acid with excellent chelating properties. These useful characteristics have lead to a growing number of patents for diverse applications in the food, pharmaceutical and detergent industries.
Autor/innen der BOKU Wien:
Haltrich Dietmar
Kulbe Klaus Dieter
Ludwig Roland
Peterbauer Clemens Karl
Zamocky Marcel
BOKU Gendermonitor:


Find related publications in this database (Keywords)
cofactor regeneration
enzyme technology
lactobionic acid
cellobiose dehydrogenase
laccase


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