BOKU - Universität für Bodenkultur Wien - Forschungsinformationssystem

Logo BOKU-Forschungsportal

Gewählte Publikation:

Bicker, W; Lämmerhofer, M; Keller, T; Schuhmacher, R; Krska, R; Lindner, W.
(2006): Validated method for the determination of the ethanol consumption markers ethyl glucuronide, ethyl phosphate, and ethyl sulfate in human urine by reversed-phase/weak anion exchange liquid chromatography-tandem mass spectrometry.
Anal Chem. 2006; 78(16):5884-5892 FullText FullText_BOKU

Abstract:
A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of the ethanol consumption markers ethyl glucuronide (EtG), ethyl phosphate (EtP), and ethyl sulfate (EtS) in human urine was developed. A reversed-phase/ weak anion exchange type stationary phase demonstrated particular suitability for the analysis of these highly polar acids. Sample preparation was minimized to centrifugation and dilution of urine prior to injection. The method was validated in the range of 5-750 mu g.L-1 with 1:20 and 1:1000 diluted urine, which corresponds to actual concentration ranges of 0.1-15 and 5-750 mg,L-1 in undiluted samples. Method validation was carried out using six different lots of human urine. Over the entire calibration range intraday and interday precision (each n = 5, three concentration levels per dilution factor) adopted values between 0.6 and 4.7% and 0.8 and 12.1%, relative standard deviation, respectively. Corresponding accuracy values ranged between 94.2 and 113.5% and 86.6 and 110.9%, respectively. Matrix effects (absolute/relative) were found to be present in minor extent (similar to-30% to +15% MS/MS signal alterations) and were well corrected by the employed isotopically labeled internal standards. The validated assay was applied to urine samples of a drinking study as well as postmortem specimens. It was possible to assess the principal potential of EtP as ethanol consumption marker. Elevated concentration levels were found in real samples; however, EtP seems to be less sensitive compared to the previously known ethanol phase II conjugates EtG and EtS.
Autor/innen der BOKU Wien:
Krska Rudolf
Schuhmacher Rainer
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
Adult -
Alcohol Drinking - urine
Biological Markers - urine
Chromatography, Ion Exchange - methods
Drug Stability - methods
Female - methods
Forensic Medicine - methods
Glucuronates - urine
Humans - urine
Male - urine
Phosphates - urine
Sulfuric Acid Esters - urine
Tandem Mass Spectrometry - methods



© BOKU Wien Impressum