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Gewählte Publikation:

Klose, V; Mohnl, M; Plail, R; Schatzmayr, G; Loibner, AP; .
(2006): Development of a competitive exclusion product for poultry meeting the regulatory requirements for registration in the European Union.
Mol Nutr Food Res. 2006; 50(6):563-571 FullText FullText_BOKU

Abstract:
Competitive exclusion treatment is able to increase the pathogen colonization resistance of day-old chicks by applying probiotic bacteria stabilizing the indigenous microflora. In order to develop a safe microbial feed additive, various bacterial strains were isolated out of the gastrointestinal tract of healthy chickens. One hundred twenty-one representatives were selected based on differences in wholecell protein patterns and screened for antagonistic properties. Five effective strains (Pediococcus acidilactici, Enterococcus faecium, Bifidobacterium animalis ssp. animalis, Lactobacillus reuteri, and Lactobacillus salivarius ssp. salivarius) exhibited in vitro the ability to inhibit a range of common pathogens and were evaluated with regard to the risks associated with genetic transfer of antibiotic resistances from animals to humans via the food chain. The probiotic strains were sensitive to several clinically effective antibiotics, though some of them showed single resistances. None of the vancomycin-resistant (R) strains carried the enterococcal vanA gene. Two tetracycline R strains were shown to harbor a tet(M)-associated resistance. The strains contained no extrachromosomal DNA and were not able to transfer the resistance by means of conjugation. On basis of the collected data the presence of easy transferable resistances was excluded and the chicken strains were considered to be suitable for the use as feed additive.
Autor/innen der BOKU Wien:
Klose Viviane
Loibner Andreas Paul
Plail Regina
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
Agriculture/legislation & jurisprudence*;Agriculture/methods*;Animal Feed/microbiology*;Animals;Anti-Bacterial Agents/administration & dosage;Bacteria*;Bifidobacterium;Chickens/microbiology*;Drug Resistance, Microbial/genetics;Enterococcus faecium;European Union;Food Microbiology;Gastrointestinal Tract/microbiology;Lactobacillus;Lactobacillus reuteri;Pediococcus;Polymerase Chain Reaction;Probiotics/administration & dosage*;

Find related publications in this database (Keywords)
competitive exclusion
defined CE product
European registration
risk assessment
Salmonella


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