BOKU - Universität für Bodenkultur Wien - Forschungsinformationssystem
Gewählte Publikation:
Häubl, G., Berthiller, F., Hametner, C., Rechthaler, J., Jaunecker, G., Freudenschuss, M., Krska, R., Schuhmacher, R.
.
(2007):
Characterization of (13C24) T-2 toxin and its use as an internal standard for the
quantification of T-2 toxin in cereals with HPLC-MS/MS.
ANAL BIOANAL CHEM, 389(3), 931-940; ISSN 1618-2642
FullText
FullText_BOKU
- Abstract:
- In this paper, the structure and the identity of fully C-13-substituted T-2 toxin were confirmed using high-resolution mass spectrometry, H-1-NMR, C-13-NMR, tandem mass spectrometry and HPLC-DAD. The purity of this compound was estimated to be at least 98.8% according to UV data. The isotopic distribution of (C-13(24)) T-2 toxin indicated a total isotopic enrichment of 98.2 +/- 1.0 atom% C-13, and the application of different MS measurement modes revealed the MS/MS fragmentation pattern of T-2 toxin. Furthermore, a stable isotope dilution mass spectrometry method for the quantification of T-2 toxin was developed using (C-13(24)) T-2 toxin as internal standard. The method was evaluated with and without conventional clean-up and validated for maize and oats. Both cereals showed strong matrix enhancement effects, which could be compensated for through the application of the isotope-substituted internal standard.
- Autor*innen der BOKU Wien:
- Berthiller Franz
- Krska Rudolf
- Schuhmacher Rainer
- Find related publications in this database (Keywords)
- T-2 toxin
- mass spectrometry
- isotope dilution
- internal standard
- maize
- oat
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