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Gewählte Publikation:

Van Hecke, W; Ludwig, R; Dewulf, J; Auly, M; Messiaen, T; Haltrich, D; Van Langenhove, H.
(2009): Bubble-free oxygenation of a bi-enzymatic system: effect on biocatalyst stability.
Biotechnol Bioeng. 2009; 102(1):122-131 FullText FullText_BOKU

Abstract:
The effect of bubble-free oxygenation on the stability of a bi-enzymatic system with redox mediator regeneration for the conversion of lactose to lactobionic acid was investigated in a miniaturized reactor with bubble-less oxygenation. Earlier investigations of this biocatalytic oxidation have shown that the dispersive addition of oxygen can cause significant enzyme inactivation. In the process studied, the enzyme cellobiose dehydrogenase (CDH) oxidizes lactose at the C-1 position of the reducing sugar moiety to lactobionolactone, which spontaneously hydrolyzes to lactobionic acid. 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt was used as electron acceptor for CDH and was continuously regenerated (reoxidized) by laccase, a blue multi-copper oxidase. Oxygen served as the terminal electron acceptor of the reaction and was fully reduced to water by laccase. The overall mass transfer coefficient of the miniaturized reactor was determined at 30 and 45 degrees C; conversions were conducted both in the reaction-limited and diffusion-limited regime to study catalyst inactivation. The bubbleless oxygenation was successful in avoiding gas/liquid interface inactivation. It was also shown that the oxidized redox mediator plays a key role in the inactivation mechanism of the biocatalysts Unobserved during previous studies. Biotechnol. Bioeng. 2009;102: 122-131. (C) 2008 Wiley Periodicals, Inc.
Autor/innen der BOKU Wien:
Haltrich Dietmar
Ludwig Roland
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
Biocatalysis -
Bioreactors -
Carbohydrate Dehydrogenases - chemistry
Disaccharides - metabolism
Enzyme Stability -
Laccase - chemistry
Lactose - metabolism
Oxidation-Reduction -
Oxygen - metabolism
Sulfonic Acids - metabolism
Thiazoles - metabolism
Water - metabolism

Find related publications in this database (Keywords)
bubble-free oxygenation
laccase-mediator system
lactobionic acid
composite membrane
cellobiose dehydrogenase


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