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Gewählte Publikation:

Marzban, G; Maghuly, F; Herndl, A; Katinger, H; Laimer, M; .
(2008): Screening and identification of putative allergens in berry fruits of the Rosaceae family: technical challenges.
Biofactors. 2008; 34(1):37-46

Abstract:
Cross-reactive proteins in small fruits of the Rosaceae family like strawberry, raspberry and blackberry revealed an unexpected complex IgE-reactivity pattern. Several copies of PR-10 and PR-14 proteins were detected by Southern blots in strawberry, raspberry and blackberry. In raspberry, the highest similarity at the DNA level for PR-10 and PR-14 (Rub i 1 and Rub i 3) was detected to strawberry sequences of Fra a 1 and Fra a 3. At the protein level, Rub i 1 and Rub i 3 showed more than 70% identity with homologous proteins of rosaceous fruits. Furthermore, raspberries contained additional putative allergens, e. g. class III acidic chitinases and cyclophilins. Blackberries were shown to share at least two well-known major fruit allergens with other rosaceous fruits, namely PR-10s and PR-14s homologous proteins. However the IgE-reactive proteins of small fruits are still not extensively investigated. The main challenges in studying small fruit allergens are the complexity of the fruit matrix, the diversity of physico-chemical properties of fruit proteins, the lack of appropriate protein extraction procedures and the missing information about the influence of processing treatments on food components.
Autor*innen der BOKU Wien:
Katinger Hermann
Laimer Margit
Maghuly Fatemeh
Marzban Gorji
Find related publications in this database (using NML MeSH Indexing)
Allergens/analysis*;Allergens/immunology;Blotting, Southern/methods;Fruit/chemistry*;Fruit/immunology;Immunoglobulin E/immunology;Rosaceae/chemistry*;Rosaceae/immunology;

Find related publications in this database (Keywords)
Rosaceous fruits
strawberry
raspberry
blackberry
IgE-reactivity
Fra a 1
Fra a 3
Rub i 1
Rub i 3
class III acidic chitinase
cyclophilin
profilin


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