BOKU - Universität für Bodenkultur Wien - Forschungsinformationssystem

Logo BOKU-Forschungsportal

Gewählte Publikation:

Leitner, C; Hess, J; Galhaup, C; Ludwig, R; Nidetzky, B; Kulbe, KD; Haltrich, D.
(2002): Purification and characterization of a laccase from the white-rot fungus Trametes multicolor
APPL BIOCHEM BIOTECH. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY; 98: 497-507.

Abstract:
The wood-degrading fungus Trametes multicolor secretes several laccase isoforms when grown on a simple medium containing copper in the millimolar range for stimulating laccase synthesis. The main isoenzyme laccase 11 was purified to apparent homogeneity from the culture supernatant by using anion-exchange chromatography and gel filtration. Laccase II is a monomeric glycoprotein with a molecular mass of 63 kDa as determined by sodium dodecylsulfate polyacrylamide gel electrophoresis, contains 18% glycosylation, and has a pI of 3.0. It oxidizes a variety of phenolic substrates as well as ferrocyanide and iodide. The pH optimum depends on the substrate employed and shows a bell-shaped pH activity profile with an optimum of 4.0 to 5.0 for the phenolic substrates, while the nonphenolic substrates ferrocyanide and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonate) show a monotonic pH profile with a rate decreasing with increasing pH.
Autor/innen der BOKU Wien:
Haltrich Dietmar
Kulbe Klaus Dieter
Leitner Christian
Ludwig Roland
BOKU Gendermonitor:


Find related publications in this database (Keywords)
Trametes multicolor
basidiomycete
laccase
polyphenol oxidase
lignin degradation


© BOKU Wien Impressum