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Gewählte Publikation:

Trashin, SA; Haltrich, D; Ludwig, R; Gorton, L; Karyakin, AA.
(2009): Improvement of direct bioelectrocatalysis by cellobiose dehydrogenase on screen printed graphite electrodes using polyaniline modification
BIOELECTROCHEMISTRY. 2009; 76(1-2): 87-92. FullText FullText_BOKU

Abstract:
Modification of graphite based screen printed electrodes (SPEs) by electrosynthesised polyaniline (PANI) has been applied to improve the electron exchange between cellobiose dehydrogenase (CDH, EC 1.1.99.18) from the ascomycete Myriococcum thermophilum and the surface of the SPE. The redox intermediate layer of the conducting polymer promotes the bioelectrocatalysis providing a higher current for lactose oxidation at a lower potential compared to CDH immobilised on a plain SPE. The current of the SPE vertical bar PANI vertical bar CDH electrode was more than 5 times higher as compared to that of a SPE vertical bar CDH electrode at a potential of 0 mV vs. Ag vertical bar AgCl. When comparing the response obtained through direct electron transfer with that obtained through mediated electron transfer. it was clearly observed that the improved current of the SPE vertical bar PANI vertical bar CDH electrode is due to the specific role of PANI, rather than caused by a rise of enzyme loading. The operational stability of the enzyme electrode based on PANI modified SPE was 5 times higher compared with that based on plain SPE. (C) 2009 Elsevier B.V. All rights reserved
Autor/innen der BOKU Wien:
Haltrich Dietmar
Ludwig Roland
BOKU Gendermonitor:


Find related publications in this database (Keywords)
Direct bioelectrocatalysis
Biosensor
Polyaniline
CDH
Lactose


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