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Hilbert, F; Mayrhofer, S; Smulders, FJ.
(2003): Rapid urease screening of Yersinia on CIN agar plates.
Int J Food Microbiol. 2003; 84(1):111-115

Abstract:: Yersinia enterocolitica is an important foodborne pathogen, but isolation of virulent Yersinia from food sources is still time consuming and requires skills. In this article, we describe a rapid urease screening on cefsulodin-irgasan-novobiocin (CIN) agar plates with an agar overlay assay. This test is simple to perform, all colonies on a plate can be checked simultaneously, it only takes minutes for detection of urease-positive colonies and the colonies survive for transfer, further characterisation, and storage. Additionally, this method is useful to isolate virulent (urease-positive and pYV harbouring) Y. enterocolitica from foodstuffs. (C) 2002 Elsevier Science B.V All rights reserved.
Autor*innen der BOKU Wien:: Mayrhofer Sigrid
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Find related publications in this database (using NML MeSH Indexing): Agar -; Carbanilides - metabolism; Cefsulodin - metabolism; Culture Media -; Enzyme Inhibitors - metabolism; Novobiocin - metabolism; Time Factors -; Urease - metabolism; Yersinia enterocolitica - enzymology
Find related publications in this database (Keywords): Yersinia; food; urease; virulence