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Gewählte Publikation:

Klose, V; Bruckbeck, R; Henikl, S; Schatzmayr, G; Loibner, AP; .
(2010): Identification and antimicrobial susceptibility of porcine bacteria that inhibit the growth of Brachyspira hyodysenteriae in vitro.
J Appl Microbiol. 2010; 108(4):1271-1280 FullText FullText_BOKU

Abstract:
Aims: To identify bacilli, lactic acid bacteria and bifidobacteria that inhibit the growth of Brachyspira hyodysenteriae. Methods and Results: A total of 80 isolates were obtained from various porcine intestinal compartments using selective conditions and grouped into 15 similarity clusters based on whole-cell protein profiles. Random amplified polymorphic DNA PCR patterns identified 24 genotypes. 16S rDNA sequencing assigned all genotypes, except eight aerobes, to established species (Bacillus subtilis, Enterococcus faecium, Lactobacillus salivarius, Lactobacillus mucosae, Lactobacillus reuteri, Lactobacillus amylovorus, Bifidobacterium thermophilum). According to their minimum inhibitory concentrations, four strains (Ent. faecium, Lact. reuteri, Lact. amylovorus, Bif. thermophilum) were susceptible to all clinically relevant antibiotics. Two lactobacilli showing multiresistance harboured the erm(B) determinant. A cross-section of eight representative strains was examined for growth suppression of two strains of Brach. hyodysenteriae, the aetiological agent of swine dysentery, and compared with intestinal strains derived from other animal sources. The Brachyspira strains were inhibited by strains of Lact. salivarius, Bif. thermophilum, Ent. faecium and B. subtilis. Conclusions: Three porcine strains of Ent. faecium, Bif. thermophilum and B. subtilis were found to be suitable as probiotic candidates because of their well-established identity, antibiotic susceptibility and antagonistic activity. Significance and Impact of the Study: For the first time, antagonistic activity of well-characterized porcine strains against Brach. hyodysenteriae is presented. These findings suggest that certain intestinal strains might have a potential as probiotic feed additives for prevention of swine dysentery.
Autor/innen der BOKU Wien:
Klose Viviane
Loibner Andreas Paul
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
Animals;Anti-Bacterial Agents/pharmacology*;Bacteria/classification;Bacteria/drug effects*;Bacteria/genetics;Bacteria/isolation & purification;Brachyspira hyodysenteriae/classification;Brachyspira hyodysenteriae/genetics;Brachyspira hyodysenteriae/growth & development*;Brachyspira hyodysenteriae/isolation & purification;DNA, Ribosomal;Gastrointestinal Tract/microbiology;Gram-Negative Bacterial Infections/microbiology*;Gram-Negative Bacterial Infections/prevention & control;Microbial Interactions/physiology*;Microbial Sensitivity Tests;Phylogeny;Probiotics*;Swine;Swine Diseases/microbiology*;Swine Diseases/prevention & control;

Find related publications in this database (Keywords)
antibiotic susceptibility
Bacillus
bifidobacteria
Brachyspira hyodysenteriae
lactic acid bacteria
porcine gastrointestinal tract
probiotic


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