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Gewählte Publikation:

Léonard, R; Wopfner, N; Pabst, M; Stadlmann, J; Petersen, BO; Duus, JØ; Himly, M; Radauer, C; Gadermaier, G; Razzazi-Fazeli, E; Ferreira, F; Altmann, F; .
(2010): A new allergen from ragweed (Ambrosia artemisiifolia) with homology to art v 1 from mugwort.
J Biol Chem. 2010; 285(35):2719-2200 FullText FullText_BOKU

Abstract:
Art v 1, the major pollen allergen of the composite plant mugwort (Artemisia vulgaris) has been identified recently as a thionin-like protein with a bulky arabinogalactan-protein moiety. A close relative of mugwort, ragweed (Ambrosia artemisiifolia) is an important allergen source in North America, and, since 1990, ragweed has become a growing health concern in Europe as well. Weed pollen-sensitized patients demonstrated IgE reactivity to a ragweed pollen protein of apparently 29-31 kDa. This reaction could be inhibited by the mugwort allergen Art v 1. The purified ragweed pollen protein consisted of a 57-amino acid-long defensin-like domain with high homology to Art v 1 and a C-terminal proline-rich domain. This part contained hydroxyproline-linked arabinogalactan chains with one galactose and 5 to 20 and more alpha-arabinofuranosyl residues with some beta-arabinoses in terminal positions as revealed by high field NMR. The ragweed protein contained only small amounts of the single hydroxyproline-linked beta-arabinosyl residues, which form an important IgE binding determinant in Art v 1. cDNA clones for this protein were obtained from ragweed flowers. Immunological characterization revealed that the recombinant ragweed protein reacted with >30% of the weed pollen allergic patients. Therefore, this protein from ragweed pollen constitutes a novel important ragweed allergen and has been designated Amb a 4.
Autor*innen der BOKU Wien:
Altmann Friedrich
Pabst Martin
Stadlmann Johannes
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
Allergens/chemistry
Allergens/genetics*
Allergens/immunology
Allergens/isolation & purification
Ambrosia/chemistry
Ambrosia/genetics*
Ambrosia/immunology
Antigens, Plant
Artemisia/chemistry
Artemisia/genetics*
Artemisia/immunology
DNA, Complementary/genetics
DNA, Complementary/immunology
Europe/epidemiology
Galactans/chemistry
Galactans/genetics
Galactans/immunology
Humans
Immunoglobulin E/immunology
North America/epidemiology
Plant Proteins/chemistry
Plant Proteins/genetics*
Plant Proteins/immunology
Plant Proteins/isolation & purification
Pollen/chemistry
Pollen/immunology*
Protein Structure, Tertiary
Rhinitis, Allergic, Seasonal/epidemiology
Rhinitis, Allergic, Seasonal/immunology*
Sequence Homology, Amino Acid



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