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Gewählte Publikation:

Warth, B; Sulyok, M; Berthiller, F; Schuhmacher, R; Fruhmann, P; Hametner, C; Adam, G; Frohlich, J; Krska, R.
(2011): Direct quantification of deoxynivalenol glucuronide in human urine as biomarker of exposure to the Fusarium mycotoxin deoxynivalenol
ANAL BIOANAL CHEM. 2011; 401(1): 195-200. FullText FullText_BOKU

Abstract:
The direct quantification of deoxynivalenol glucuronide (DON-GlcA) by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and its application as a biomarker of exposure to the Fusarium mycotoxin deoxynivalenol (DON) is reported. Usually, DON exposure is estimated from dietary average intakes or by measurement of the native toxin in urine after enzymatic hydrolysis with beta-glucuronidase. These methods are time-consuming, expensive, and fail to determine the ratio of DON to DON-GlcA in a simple one-step procedure. One of the main reasons for the use of indirect methods is the unavailability of DON-GlcA standards. Consequently, DON-3-O-glucuronide (D3GlcA) was synthesized and used to develop a method allowing quantification of both DON and D3GlcA by a simple "dilute and shoot" approach without the need for any cleanup. Limit of detection and apparent recovery of D3GlcA was 3 mu g l(-1) and 88%, respectively. The identity of D3GlcA in human urine was confirmed by comparison with LC-MS/MS measurements of the synthetically produced D3GlcA standard which was also used for external calibration. The applicability of the method was demonstrated through the analysis of urine samples obtained from a volunteer during regular and cereal-restricted diet, respectively. In regular-diet urine samples, D3GlcA was quantified in concentrations > 30 mu g l(-1) by this approach.
Autor*innen der BOKU Wien:
Adam Gerhard
Berthiller Franz
Krska Rudolf
Schuhmacher Rainer
Sulyok Michael
Warth Benedikt
BOKU Gendermonitor:


Find related publications in this database (Keywords)
Deoxynivalenol glucuronide
Mycotoxin
Biomarker
Exposure assessment
Human urine
LC-MS/MS


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